Functional Analysis Of Tfiiia Type Zinc Finger Protein Gene Zfp150from Rice(Oryza Sativa L.)

Zinc finger protein is one of the transcription factors and function in the prosssess of development and stress response in arabidopsis, petunia and rice. Huang identified a TFIIIA type zinc finger gene from japonica rice landrace Jiucaiqing using the strategy of RT-PCR and the protein is proposed of144amino acids with a predicted molecular weight of15KDa. This gene was named as ZFP150based on the molecular weight. ZFP150has two classical and plant-specific QALGGH motifs in the zinc finger region but lacking DLN-box in the C-terminal, and a putative nuclear localization signal (NLS) at its N-terminus. The researches in this thesis were accomplished based on this background and some results are given as follows.The analysis of gene sequence shows that the promoter of ZFP150has many DREB factor and ABRE element and several TFs binding element. The trsnacriptional activating assay in yeast shows that ZFP150doesn’t have the ability of transcription activity. When the LLELF-motif in the C terminal of ZFP150was cut, the protein still cannot activate the expression of reporter genes, so we speculate that ZFP150may function as a repressor and has other transcription repress region. In the recombination E.coli, we have gained the ZFP150special protein from the prokaryotic expression. Tissue expression analysis revealed the expression of ZFP150was in a low level in the whole plant but the flowering spike. Further expression analysis revealed ZFP150was induced by salt, cold, drought and ABA with different patterns. It may take part in many pathways of stresses response. We have constructed transgenic sense and antisense vectors for furthermore experiment.