Preliminary Analysis On The Genome Of Paenibacillus Mucilaginosus3016

Paenibacillus mucilaginosus is consequently widely used as microbial fertilizer in agriculture. It can release soluble content of K, P and other beneficial elements in the soil, promote crop in growth, increase yield and improve quality of crops, etc. So, it has attracted considerable attention of researchers and as a focus and hot spot in the field of microbial fertilizers. P. mucilaginosus3016, which was isolated from rhizosphere soil, was a multi-function strain, such as phosphorus-solubilizing, potassium-releasing, nitrogen-fixing and phytohomone-producing etc.P. mucilaginosus3016genome was determined by Roche454Pyrosequencing and Illumina Sequencing-by-Synthesis. The results showed that, the complete genome sequence of3016strain is a circular8,739,048-bp chromosome with mean GC contents of58.3%. There were7057coding genes,42rRNA operons, and170tRNAs in the chromosome. No single plasmid was detected.The two chains of P. mucilaginosus3016genome both coded ORFs, with positive strand DNA coding3366ORFs and negative strand DNA coding3691ORFs. According to the standard of COGs, the genes of P. mucilaginosus3016could be divided into23function groups,1unknown functional group and1hypothesis functional group. There were170tRNA genes including20kinds of amino acides transportation. The20kinds of amino acides mentioned above had specific codens and8kinds of them had more than one codens. The tRNA of the same kind amino acid was certain biased in the use of bases. Meanwhile, this phenomenon also reflected the wobble of codens. Comparative genomics was followed, the comparison of genome among P. mucilaginosus3016, P. polymyxa SC2, Paenibacillus sp. JDR-2and P. mucilaginosus KNP414investigated the linearity between structure of chromosome and distribution of gene. The investigation indicated that, the linearity among the nucleic acids levels of3016, P. polymyxa SC2and Paenibacillus sp. JDR-2was not obvious. The length of homogeneous nucleic acid segments was shorter than5k and there is no definite distribution discipline. However, there was obvious colinearity between P. mucilaginosus KNP414and P. mucilaginosus3016.6712CDSs out of the total7317in P. mucilaginosus3016could be found the corresponding homogenous protein in P. mucilaginosus KNP414with a percentage of92.13%;3496CDSs could be found the corresponding homogenous protein in P. potymyxa SC2with a percentage of47.99%;4676CDSs could be found the corresponding homogenous protein in Paenibacillus sp. JDR-2with a percentage of64.19%. According to the principle that the length of gene is greater than40%of comparative region and the similarity percentage is above40%,41nitrogen fixation genes were found in P. mucilaginosus3016genome, such as nif A and nif L, but nif H waa not found.On the existing experiment conditions, the nitrogenase activity was not detected in P. mucilaginosus3016. The nitrogen metabolic pathways showed that the strain could not change N2to NH4+-N, and had not a full range of nitrogenase genes. The three results mentioned above were consistent. Later on, total nitrogen content of P. mucilaginosus3016fermented liquid was determined by Kjeldahl method. And15N isotope-labelling method was followed. The both results verified the nitrogen fixation of P. mucilaginosus3016. The contradiction of the conclusions may be attributed to the inappropriate condition of Acetylene reduction method or the unverified nitrogen fixation function genes in2791genes with unknown and hypothetical functions, which would be analyzed later.This was first genome sequence deciphering of P. mucilaginosus, and may be a great breakthough in the investigation process. The research about the genome information of P. mucilaginosus3016was important to its application in agriculture and industry, and provided with substantial statistical and the original principles for Functional Genomics, Proteomics, expression and regulation of functional genes, etc.