| China’s ruraL areas have Large popuLation and wide variety of biomass energy.But the use of Livestock manure and other biomass energy is not sufficient by farmers.A Lot of farmers directLy disposed Large number of unprocessed manure into the environment, even as a crop fertiLizer, farmers does not do any handLing for Livestock manure. But in practice, it was found that above approaches wiLL not onLy waste of resources but aLso easiLy Lead to animaL infectious disease, infectious diseases to both humans and animaLs and parasitic diseases. Moreover, there are Large amounts of nitrogen, phosphorus, potassium and some heavy metaL eLements in Livestock manure, if the manure can not be adequateLy handLed, they wiLL easiLy cause water, air and soiL poLLution and be harmfuL to human heaLth. So peopLe shouLd be based on the actuaL situation of the ruraL deveLopment to resoLve the probLems of ruraL Livestock manure poLLution, to deveLop the technoLogy of anaerobic methane fermentation using animaL manure and to product high quaLity and green energy.This is one of the urgent probLems which need to be soLved in the of the process ruraL deveLopment.In this experiment, we preLiminary grasped the succession and changes in number of dominant fLora of pig manure and cow dung through understanding and mastering changes of the physicaL and chemicaL properties of fermented raw materiaLs in various stages of fermentation process. The test anaLyzed the reLationship between such change and the anaerobic methane fermentation, In order to according to the characteristics of the various stages of methane fermentation to estabLish microbiaL controL methods to improve biogas production and maintain the stabLe of methane fermentation system. At the same time, it was found that anaerobic methane fermentation can be carried out in darkness, but we aLso need further study about whether and how anaerobic methane fermentation was infLuenced by the iLLumination. So the experiment researched the effect of iLLumination on pig manureã€cow dung’anaerobic fermentation and monitored in different iLLumination conditions the changes of totaL aLkaLinity, voLatiLe fatty acids, pH, NH3-N of pig manure, cow dung in the process of fermentation.At35℃,experiments use pig manure and cow dung as fermentation raw materiaLs for anaerobic methane fermentation. At the same time, experiments research the microbiaL popuLation change of pig manure and cow dung in the fermentation process and study on effect of iLLumination intensity on pig manureã€cow dung ’anaerobic fermentation. The resuLts showed that:It is simiLar that the succession of dominant bacteria in the manure, cow dung in different stages of fermentation. There is a significant correLation between the succession and Decomposition, utiLization and transformation of the fermentation materiaL. In initiaL of fermentation, aerobic microorganisms were the main groups to cause the transformation of organic matter and aerobic acid-producing bacteria were the dominant fLoras.The quantities of pig manure, cow dung’s acid-producing bacteria were1.5×107per miLLiLiter and1.1×107per miLLiLiter, respectiveLy. SubsequentLy, due to the consumption of oxygen in fermentation tank by aerobic microorganisms, the increase of reducing substances and the decrease of oxidation-reduction potentiaL so the number of aerobic bacteria dramaticaLLy reduced, by contrast, the number of anaerobic bacteria graduaLLy increased. The period of gas production, pig manure, cow dung’s methane bacteria were respectiveLy9.5×106per miLLiLiter and1.1×107per miLLiLiter. In the group of anaerobic, the added rate of aerobic acid-producing bacteria was greater than that of magnifications bacteria. Therefore, in initiaL of fermentation, the pre-dominant bacteria were aerobic microorganisms, then anaerobic bacteria quickLy raised, but no matter those were aerobic microorganisms or anaerobic microorganisms, the added rate of aerobic acid-producing bacteria was greater than that of magnifications bacteria. So the test enLightened peopLe according to the characteristics of the various stages of methane fermentation to estabLish microbiaL controL methods to improve biogas production and to maintain the stabLe of methane fermentation system.The cumuLative gas production of pig manure under different iLLumination conditions, at0hours (P1),6hours (P2),12hours (P3),24hours (P4), is8136mL,857mL,15618.5mL, OmL respectiveLy. P3>P1>P2>P4.The cumuLative gas production of cow dung under different iLLumination conditions, at0hours (C1),6hours (C2),12hours (C3),24hours (C4), is3182.5mLã€1244.5mLã€7680mLã€567.5mL respectiveLy,C3>C4>C1>C2. In the same iLLumination conditions, cumuLative gas production of pig manure and cow dung were:8136mL (P1)>3182.5mL(C1),857mL (P2)<1244.5mL (C2),15618.5mL (P3)>7680mL (C3),0mL(P4)<567.5mL (C4).ResuLts indicated that there was a great deaL difference in terms of the same fermentation materiaLs’gas production under the different iLLumination intensity. In addition, gas production of different fermentation materiaL, at the same iLLumination intensity, was different. So peopLe shouLd seLect raw materiaLs according to iLLumination conditions so that to improve gas production.According to SPSS17.0, it was found that under different Light conditions, there was dynamic of reLationship among cumuLative biogas yieLd of pig manure and cow dung and totaL aLkaLinity, voLatiLe fatty acids, pH, NH3-N. We found that the infLuence coefficient of these constraints changed with the change of iLLumination conditions by comparison a higher gas production rate and cumuLative gas production. Gas production of pig manure and cow dung was a negative correLation with VFA, was a positive correLation with pH. TotaL aLkaLinity was a negative correLation with the P2and a positive correLation with P3. NH3-N was negativeLy correLated with the C4and a positiveLy correLated with C3. |