| Potassium is one kind of the mineral nutrition essential for rice growth and development. In our country, the potassium mineral resources is extreme deficiency, as well the arable soil is generally potassium shortage, so, the condition of potassium malnutrition during rice production has come to be a principal limiting element for China’s rice development and production. To cultivate the rice that can maintain higher yield even on lower potassium levels, is an effective way to alleviate the lack of China’s potash supply, also help to reduce the pollution of Natural water body environment during the rice production. In this study, the12Potassium nutrition efficiency gene was transformed to rice variety zhonghuall in order to improve low potassium stress tolerance in rice by using agrobacterium-mediated transgene technique.The main results are as follows:1. The12Potassium nutrition efficiency gene which were carried by plant expression vector pCUbi1390/gene, were delivered into the calli induced from the mature embryo of zhonghuall by Agrobacterium-mediated transformation. Each gene transformation obtains8-36transgenic plants and finally we received more than300regeneration plants.2. PCR analysis of Hygromycin resistance gene and target gene showed that the positive rate was100%,and preliminarily determined that the target gene had been integrated into the rice genome.3. In the process of low potassium stress, biomass of TO and T1transgenic plant was higher than non-transgenic control, Low potassium-resistant features of the plants that transferred different potassium gene are also differences. Transgenic T1plants of AtKATl had the highest plant height, OsAKT4was followed; Transgenic T1plants of C2had the longest root length, OsHKT4-eGFP was followed; Transgenic T1plants of OsAKT4had the heaviest fresh weight, Os(MD+kd) was followed. So, transgenic plants of Os(MD+kd) and OsAKT4had more resistant to low potassium among these12genes. |
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