The Study On The Role And Effects Of AMF In Iron Tailings Reclamation Of Inner Mongolia Grasslands

A pot culture experiment was carried out to study the influence of inoculating arbuscular mycorrhizal fungi (AMF) including Glomus mosseae and Glomus versiforme on growth, arbuscular mycorrhizal colonization, mineral nutrient uptake, and heavy metal uptake or translocation by four native grasses species, Agropyron cristatum (L.) Gaertn., Elymus dahuricus Turcz., Astragalus adsurgens Pall. and Medicago sativa L., grown on iron mine tailings overlying soil in grassland ecosystem in Inner Mongolia, China. A greenhouse experiment was conducted to evaluate the potential role of arbuscular mycorrhizal fungi Glomus mosseae and Glomus versiforme in encouraging revegetation of iron mine tailings. Three gramineous plant species Zea mays L., Sorghum bicolor (L.) Moench, Triticum aestivum L., together with a leguminous plant Glycine max (L.) Merr. associated with and without AMF were grown in Fe mine tailings to assess mycorrhizal effects on plant growth, nutrition status and heavy metals accumulation and translocation. The results showed that inoculation with G. versiforme could make the dicotyledonous A. adsurgens and M. sativa which couldn’t live on the iron mine tailings survive. In iddition, inoculation with G. versiforme increased the biomass of A. cristatum and E. lahuricus by33%and87%compared to the control. Inoculation with G. versiforme ignificantly (ⅰ) increased N, P and K concentrations and contents (ⅱ) decreased C:N: ratios (iii) inhibted Zn and Cu translocation of E. dahuricus. In addition, the results howed that symbiotic associations were successfully established between AMF, in (articular G. versiforme and four crop species, and the average percentage of nycorrhizal colonization exceeded60%. Compared to the control, inoculation with G. ersiforme significantly (ⅰ) increased N, P and K contents (ⅱ) decreased C:N:P ratios iii) inhibted Cu translocation of Z. mays. The experiment provided evidence for the (?)otential use of local plant species in combination with G. versiforme for ecological estoration of iron mine tailings in Inner Mongolia grassland, China.A greenhouse experiment was conducted to screen the potential role of rbuscular mycorrhizal fungi Glomus mosseae, Glomus etunicatum, Glomus ntraradices and Glomus aggregatum in encouraging revegetation of iron mine filings. Two plant species Zea mays L. and Glycine max (L.) Merr. associated with nd without AMF were grown in Fe mine tailings to assess mycorrhizal effects on lant growth, nutrition status and heavy metals accumulation and translocation. The ssults showed the mycorrhizal effectiveness of the G. intraradices was the highest, nd the inoculation treatments with G. aggregatum produced the most biomass. noculation with G. aggregatum increased the total dry weight of Z. mays by288%ompared to the control treatments. Furthermore, inoculation with G. aggregatum ignificantly (ⅰ) increased N, P and K contents (ⅱ) decreased C:N:P ratios (ⅲ) inhibted Fe, Mn, Al and Cu translocation of Z. mays. The experiment result indicates that the G. aggregatum can be selected as a candidate used in the cover reconstruction of iron mine tailings in Inner Mongolia grassland, China.