| In the growing season (from June to October) of2010, take the primitive korean pine forest of Liangshui Nature Reserve in Heilongjiang province as the research object, the canopy closure stand as a comparison, collect the soil at0～20cm in different directions of three different size of gaps. The purpose is to reasarch the spatial heterogeneity of carbon and nitrogen characteristics of litter and soil, and the soil microbial, in korean pine broad-leaved mixed forest gap, which located in Xiaoxing’anling mountain of northeast region in China, and investigate how do them response for the size of gap and sample location with seasonal change. The results are as follows:1. Gap size and sample location have no significant effect on the organic carbon and total nitrogen content of litter. The organic carbon content of litter is between394.68g/kg and639.48g/kg, reaching its peak in September and valley value in August. The total nitrogen content is between16.61g/kg and25.58g/kg, when the former is at June and the latter at September, both appearing at the east. The total nitrogen content of litter in all six sample directions in June and July is lower than the other months. And the litter total nitrogen content within canopy closure stand was lower than in the gaps. The C/N of litter is between18.34and30.44, when the former belongs to the centre location in the gap and the latter belongs to the canopy closure stand, meaning that the litter decomposition is fastst in the gap centre and slowest in the canopy closure stand. Totally, C/N of litter reach its valley value in July, meaning that the decomposition is fastst at this month.2. Gap size and sample location have significant effect on the organic carbon of soil. The soil organic carbon content in gap is between96.44g/kg and176.32g/kg. An the same direction, The soil organic carbon content of the sampling point in the canopy closure stand is higher than the one in the gap, meanwhile higher than the centre sample points. The total nitrogen content of soil is between6.24g/kg and11.71g/kg. There are six sample points in nine reach their peak of soil total nitrogen content in October, when eight sample points reach the valley value in September. Except for the north shaf, total nitrogen content of the sampling point in the canopy closure stand is higher than the ones in the gap at the other three directions. The C/N of soil is between12.41and19.71. The C/N of all sample points reach its peak in August. Except for the east shaf, C/N of the sampling point in the canopy closure stand is higher than the ones in the gap at the other three directions. The ammonium nitrogen is between1.94mg/kg and11.76mg/kg, when the nitrate nitrogen is between7.42mg/kg and46.79mg/kg.3. Sampling theorem has significant discrepancy on soil bacteria and total microbial, has very significant difference on fungus and actinomycete. Gap size has very significant difference on soil bacteria, total microbial, fungus and actinomycete. Sampling location has not significant discrepancy on soil bacteria and total microbial and fungus, but has very significant difference on actinomycete. In different size gap, every kind of microbial didnot reach their peak at the same time. The number of soil bacteria has great change with the time goes on. The soil fungus has the least quantity at the gap centre, with the following is the canopy closure stand. The order of actinomycete is gap centre> east> west> north> south. Different kinds of microbial reach its peak at differrent time. At different sampling time, in different size of gap, at differrent sampling direction, soil bacteria, fungus, actinomycete and total microbe, have a approximate variation trend. |
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