Detection Of QTL For Agronomic And Qualitive Characterizations In Wheat Using DH And Immortalized F₂Populations

168doubled haploid （DH） lines deriving from a cross between Huapei3and Yumai57,and an immortalized F₂（IF₂） population generated by randomly permutated intermating ofthese DHs were investigated, and QTL for heading date, flag leaf,1000-kernel weight, grainlength, hardness and grain protein content were detected. The work would provide generesources for molecular marker assisted selection and molecular aggregation breeding, andsupply information for marker-assisted selection strategy in hybrid wheat breeding.The results were as the following:1. The genetic map was constructed based on368marker loci, which covered a total lengthof3074.1cM with an average distance of8.35cM between adjacent markers in thegenome.2.168doubled haploid （DH） lines deriving from a cross between Huapei3and Yumai57,and an immortalized F₂（IF₂） population generated by randomly permutated intermatingof these DHs were investigated. The immortalized F₂population is favorable for QTLanalysis due to its similar proportions of genotypes in the population, and the replicabilityat multiple locations over several years.3. The QTL for heading date in both DH and IF₂population. The QTL Qhd5D for HD waspersistently expressed in both of DH and IF₂population, and the contributions of additiveeffect in DH and IF₂population were between26.49%-55.45%. The QTL can increase0.79-2.34days for heading date.4. The QTL for Flag leaf in DH and IF₂population. The QTL Qfll2A and Qfll3A for Flagleaf lengh was persistently expressed in both of DH and IF₂population, and thecontributions of additive effect in DH and IF₂population were between7.64%-23.96%and7.33%-13.41%. The QTL Qflw5B for Flag leaf width was persistently expressed atboth of DH and IF₂population, and the contributions of additive effect in DH and IF₂population were between8.40%-18.17%. The QTL Qfla7D for Flag leaf area waspersistently expressed at DH population, and the contributions of additive effect wasbetween6.18%-23.22%. The QTL Qfla3B for Flag leaf area was persistently expressedat IF₂population, and the contributions of additive effect was7.69%and4.24%indifferent environment.5. A total of32additive QTLs and18pairs of epistatic QTLs were detected, including8additive QTLs and5pairs of epistatic QTLs for1000-kernel weight,10additive QTLsand6pairs of epistatic QTLs for grain length,10additive QTLs and6pairs of epistatic QTLs for grain diameter, and7additive QTLs and1pair of epistatic QTLs for grainhardness. The QTL Qtkw6A for GW was persistently expressed in both of DH and IF₂population, and the contributions of additive effect in DH and IF₂population were9.39%and11.75%, respectively, and1.37%for that of dominance effect. The QTL Qgd6A forgrain diameter was persistently expressed in both of DH and IF₂population, and thecontributions of additive effect in DH and IF₂population were respectively15.02%and15.03%, It was the same as the QTL Qgl6A, the contributions of additive effect in DHand IF₂population were respectively14.96%and15.10%.6. The QTL Qgpc3B for grain protein content was persistently expressed at IF₂population,and the contributions of additive effect was between5.32%-6.21%in differentenvironment.