Development Of A Heat-Resisitant Newcastle Disease (La Sota) Live Vaccine

Avirulent live vaccine against New castle disease(ND) is the most widely used vaccines in avain industry.At present, conventional stabilizers used in live vaccine in our country are composed of sugar, skimmed milk, glutin and so on. Because of simple combination of conventional stabilizers and poor heat-resistant protection, the live vaccines only preserved3-6months in2-8℃, so, it commonly preserved in-15℃.However, equipments in cold chains restrctedlong-distance transfer and long time and shortage of storage of live vaccine, which made efficiency of live vaccine easily lost. New forms of heat-resistant lyophilized stabilizers have been applied in live vaccines in developed country, the live vaccines preserved24months in2-8℃. Because of short storage term for lyophilized live Newcastle disease vaccine of in2-8℃, exploring the new forms of lyophilized heat-resistant stabilizers is of necessity.Three groups heat-resistant stabilizers were developed in therst round test, and tested in production of live vaccine of NDV La Sota. In the tests of storage term for Cl,C2and C3, store for7and10days at37℃were reduction in the three test groups respectively.EID50of the best protection was reduced100.6an d101.0. In the tests of storage term for C1,C2and C3, store for30days at25℃.There were101.4and101.1, respectively, reduction in the C1and C2test groups. EID50of the best protection was decreased to100.7.In the tests of storage term for C1,C2and C3, store for3and5days at45℃, results showed protectan combination C3was ideal for use.Four batches of propels adjuvant vaccine to Newcastle disease ND that were made from three kinds of virus material were produced and the preservation of them were also evaluated in this study. In the tests of storage term for three groups, store for30days at25℃-3O℃,10days at37℃,5days at45℃,10min at100℃. EID50of the group of37℃was decreased to105.8. EID50of the other groups were reduced100.6、103.5-3.9、104.5-4.9respectively.Only a little property chang when production store at2-8℃for9,12.15.18.21.24.27months. In a word,this trial has been proved that The results indicated stabilizers of C3was of good heat-resistance and showed promise in application of production the conservative period of the vaccine is24months at2-8℃.We are registered under the new reporting requirements of veterinary drugs supplement the kinds of research and test drug in2005.wo won the new national veterinary certificate in2008.