Analysis The Fungal Population Within Morels Growth Soil In Yunnan Province

Morehella esculenta is a famous edible and medicinal fungi, and have broad prospect for development and utilization. Morels artificially cultivation methods have not obtained effective breakthrough until now, however, a key step in the half ariticailly cultivation procedure is the addition of casing soil with beneficial microorganisms which can stimulate morels to produce fruit body. Therefore, it may be a convenient way in Morels successfully cultivation to find out the beneficial microorganisms and imitate the wild environments.First, The research focused on the fruiting body of morels, We isolated the fungi from the fruiting body of morels, hope find some fungi has certain functions to host’s growth. After that, The research focused on ecology of morels. Fungal community, revealed by the28S rDNA clone libraries of the six soil samples. We hope to find some dominant fungal which make for the development of the morchella fruit bodies. And then we comparing the dominant fungal groups in the soil samples with the isolated fungal from the fruiting body of morels.We hope this work will provide a useful reference for the future research in Morels artificially cultivation and other fields. The main research works were discussed below:1. we had isolated39fungi from four fruiting body of morels. ITS region of39strains were amplified by PCR. The result indicated that the length of entire ITS of33strains was738bp, Comparisons with sequence datas in GenBank revealed that they belonged to Morchella conica; the length of entire ITS of the others was less than738bp, they belonged to other category:the "3" strain belonged to Talaromyces trachyspermus, the "5""23"、"37" belonged to Botryotinia fuckeliana,the "6"strain and "24"strain belonged to Cordyceps sinensis and Mortierella alpine.2. Obtaining high quality total DNA from the soil samples,28S rDNA region were amplified by PCR, after that set up28S rDNA clone libraries of the above six soil samples, DNA sequencing of the dominant clones(the quantity≥5), The results showed that:In the morels growth soil, the sequenced fungi belonged to Pezizomycetes、Sordariomycetes; some strains belonged to Chytridiomycetes; Basidiomycota; zygomycotina. Comparison of the diversity indices of four libraries indicated:the value of the four libraries is close to each other.comparied fungal communities inhabiting Morchella growth soil(XR-5) with common soil(XR-4) revealed there are many differences between XR-4and XR-5. we collected soil samples(XR-5、XR-2) from the same place for two consecutive years, comparion of the dominant fungal groups of XR-5and XR-2indicated large differences in the community composition. Fungal communities associated with XR-5soil were much less varied in composition compared with those associated with XR-2soil. Besides the population of the Morchella conica, in the soil is relate to the yield of morels.3. the "6" strain isolated from the fruiting body of morels found in the D library, the value of the abundances is1.69%; the "24" strain isolated from the fruiting body of morels found in the A library, the value of the abundances is4.88%; the "5、23、37" strain isolated from the fruiting body of morels found in the A library, also exist in the C library. The three strains may had certain functions to host’s growth. But Now, the processes involved in the formation and development of mushroom primordial remain unclear. The roles of the fungal communities inhabiting the casing soil, on the development of the morchella fruit bodies requires further study.