The Trans-Generational Effect Of Fenvalerate On The Reproductive Capacity In Mice

To clearify the Fenvalerate (Fen) effective doses of trans-genernational effect on the reproductive capacity in mice.40KunMing male mice were evenly divided into4groups. The mice from each group were daily gastric perfused with0,0.5,10,100mg/kg-BW of Fen respectively. After7days treatment, all mice were sampled. The sperm counts, sperm motility and spermic abnormality rate were evaluated, the ultrastructure of mice testis was observed by transmission electron microscopy. The genomic DNA of testis were isolated and the DNA methylation level of imprinting control region of Igf2/H19were checked.To evaluate the trans-generational effects of Fen on the reproductive capacity in mice, eleven Kun Ming male mice were daily gastric perfused with10mg/kg·BW of Fen. After40days perfused, mice were mated with normal female mice, and delieved first generation (G1). And when the F1mice matured, breeded the G1mice as follow, Control G1♂×Control G1♀(Group Ⅰ, control), Control♂×G1(Group Ⅱ), G1♂×Control G1♀(Group Ⅲ), G1♂×G1♀(Group Ⅳ), and yielt second generation (G2). At the age of60days, mice from Gland G2were randomly sampled.(1) The microstructure of uterine and ovary, the levels of serum Progesterone (P) and Estradiol (E2) of G1female mice were analyzed.(2) The levels of serum Testosterone (T) and E2of G1male mice were analyzed.(3) The microstructure of uterine and ovary, the levels of serum P and E2of G2female mice were analyzed.1. The effects of Fen on the reproductive capacity in male miceThe results showed that the sperm counts from all treatments were significant lower than the control (P<0.01),10mg/kg·BW and100mg/kg·BW were lower than0.5mg/kg·BW (P<0.05); The sperm motility from all treatments were significant lower than the control (P<0.01),100mg/kg·BW was significant lower than0.5mg/kg·BW and10mg/kg·BW (P<0.01). The spermic abnormality from all treatments were significant higher than the control (P<0.01), and the spermic abnormality increased as the Fen dose increased. The acrosomal integrity of spermatozoa in the groups of10mg/kg·BW and100mg/kg·BW were significant higher than0.5mg/kg·BW and the control (P<0.01).Spermatogenetic cell in groups of10mg/kg·BW and100mg/kg·BW were significant lower than0.5mg/kg·BW and the control (P<0.01), no statistic difference between0.5mg/kg·BW and the control. Spermatogenic cells and sperms in the seminiferous tubules were desquamated in the Fen exposed mice,10mg/kg·BW and100mg/kg·BW were significant lower than0.5mg/kg·BW and the control (P<0.01), there was no statistic difference between0.5mg/kg·BW and the control. Leydig cell in groups of100mg/kg·BW was significant lower than the other groups (P<0.01), there was no difference in the other three groups. Reduced sperm10mg/kg·BW and100mg/kg·BW were significant lower than0.5mg/kg·BW and the control (P<0.01), there was no difference between0.5mg/kg·BW and the control. Ultrastructure analysis obviously founded swelled, vacuolized mitochondria, more lysosome, and expanded endoplasmic in the sertoli cells in the treatment but not in the control, and the phenomena increased as the the Fen dose increased.2. G1gonad histological analysis and gonadal hormones analysisAs for the uterine and ovary structure of G1in estrous period, the epithelium mucosae of the treatment was significant thicker than the control group (P<0.01), the thickening of endonetrium, the corpus luteum, primodial follicle, primary follicle, secondary follicle, mature follicle, atretic follicle didn’t show statistic difference with the control. In diestrous, the corpus luteum of the treatment was significant bigger than the control group (P<0.01), the epithelium mucosae, the thickening of endonetrium, primodial follicle, primary follicle, secondary follicle, mature follicle, atretic follicle didn’t show statistic difference with the control.In estrous period, the levels of serum P of the treatment group was significant lower than the control (P<0.05), but the treatment was significant higher than the control in diestrous (P<0.01). The levels of serum T, the treatment was significant higher than the control (P<0.01).3. G2gonad histological analysis and gonadal hormones analysisIn diestrous, the results of histological analysis of G2female mice ovarine didn’t show statistic difference among the four groups, the endonetrium of group IV was significant thicker than the other three groups (P<0.05), but the mucosae of endonetrium were no difference in four groups.In diestrous, the levels of serum P in Group Ⅱ, Ⅲ and Ⅳ was lower than the control (P<0.05), the group Ⅱ and Ⅲ were higher than the group Ⅳ (P<0.05), the levels of serum E2in Group Ⅱ,Ⅲ and Ⅳ was higher than the control (P<0.05).4. The DNA methylation level of imprinting control region of Igf2/H19Fen significant increased the DNA methylation level of imprinting control region of Igf2/H19,0.5mg/kg·BW,10mg/kg·BW and100mg/kg·BW were significant higher than the control (P<0.05), but no statistic difference was found in the groups of0.5mg/kg·BW, lOmg/kg·BW and100mg/kg·BW.In conclusion,(1) Fen severly influence the semen quality in adult male mice, this influence was does-dependent.(2) The effect of Fen to male reproductive capacity can transmit to its offspring, verify the offspring gonads histomorphology, sexual hormone secretion, and the sexual cycle. And this phenomenon at least can transmit to two generations.(3) Fen increased the DNA methylation ratio of imprinting control region of Igf2/H19.