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Application And Study On Sanolife MIC-F For Cultivating Cynoglossus Semilaevis Günther Larvae And Scophthalmus Maximus Larvae

Posted on:2013-03-06Degree:MasterType:Thesis
Country:ChinaCandidate:M M KangFull Text:PDF
GTID:2233330377452847Subject:Fisheries
Abstract/Summary:PDF Full Text Request
Flatfish species are high-level waters fish species with high economic value,high-proteinand high nutrient levels. They are expensive but favored by domestic and foreign consumers.The number of this type of fish is huge. Scophthalmus maximus and Cynoglossus semilaevisGünther is the main type of factory farming fishes.They are established meritorious serviceto lead our marine fish onto the road of factory farming. With the rapid industrialdevelopment and the intensive level of aquaculture which bring with a large number offishery drugs and chemicals, the environmental pollution is increasingly serious.It hasbecome the most serious problem which influences flatfish species during the development.The use of traditional antibiotics and chemical drugs has caused an increasingly number ofadverse effects in resistant strains. Fish organ in pharmaceutical damage and drug residuesdestoried the ecological environment and many other adverse consequences lead to exportbarriers. Therefore, sanolife MIC-F, which takes the place of antibiotics and chemical drugsin "green culture", became a major demand of the state in recent years and caused thewidespread concern of the aquatic community.In order to study the effect of the growth status and water quality factors of Scophthalmusmaximus for Scophthalmus maximus larvae and the Cynoglossus semiloevis Güntherlarvae,the experimental design are as follows: Cynoglossus semiloevis Günther andScophthalmus maximus broodstock first after spawning and fertilization, artificial incubation,and then pick smooth and transparent, oil the ball morehomogeneous homologous eggs9000,the average devoted to the six ponds. The test set do not add sandnolife MIC-F threebreeding pools (A1, A2, A3) for the test group, add sandnolife MIC-F three breeding pools(B1, B2, B3) was the control group for each treatment, three parallel testeach breeding poolsas a parallel unit. From breeding the first four days (after the opening of the fry) to addSanolifeMIC-F (a mixture of probiotic powder, produced by Tianjin Yingwei Aquatic Products Co., Ltd., the composition of Bacillus spores cocentration of5×1012CFU/kg),before this test group and the control group using the same approach. The experimentalgroup was fed add rotifers and Artemia SanolifeMIC-F, the control group was fed ahomogeneous source of rotifers and Artemia. The fish is fed twice a day.The time is9:00and17:00and the day feeding rate is4percent to6percent of of fish weight. Observe the fishfeeding state and adjust the feeding amount each time. The experimental observation periodare42days and40days respectively. During the feeding experiment,the author measured therate of weight gain, growth rate, fatness, survival rate, as well as sandnolife MIC-F of thehalf-smooth tongue sole and turbotin and aquaculture process water pH, T, DO, ammonianitrogen, nitrite nitrogen, also calculated the economic benefits of two fishes, theexperimental conclusions are summarized as follows:1. Trials of SanolifeMIC-F feeding Cynoglossus semilaevis Günther, under the same feedingand management conditions,the weight gain rate of the experimental group than the controlgroup, increased by7.31%~7.48%, P<0.05, the difference is very significant.The Fatnessof experimental group than the control group increased by5.29%~5.41%, P<0.05,thedifference is very significant.The survival rate of experimental group than the control groupincreased by5.10%~5.67%, a significant difference between the experimental group thanthe control group, the growth rate increased by2.03%to2.33%, P<0.05, the difference wassignificant. That under the experimental conditions, adding sandnolife MIC-F on theCynoglossus semilaevis Günther the weight gain rate, growth rate.,fatness, and survival ratehave a significant role in promoting and significant economic benefits.2. After feeding sandnolife MIC-F in the Cynoglossus semilaevis Günther breeding process,the water quality parameters in the following changes: the pH change of the test group andcontrol group is P=0.164>0.05, the difference is not significant; the DO values change oftest group and control group is P=0.5318>0.05, the difference is not significant;thetemperature variation of the experimental group and control group is P=0.596>0.05, thedifference not significant;the ammonia change of experimental group and control group is P=0.447>0.05, no significant difference; the difference of sub-nitratenitrogen change of testgroup and control group is not significant. In this experiment, add sandnolife MIC-F on thepH, DO, temperature, ammonia, nitrite did not significantly affect the reason may be insufficient to achieve the decomposition of the concentration of pollutants due to theconcentration of Bacillus. In this experiment, add sandnolife MIC-F on water quality,although not purification, but also no adverse effects.3. Trials of sandnolife MIC-F feeding Scophthalmus maximus, in the same feeding andmanagement conditions, weight gain rate of the experimental group than the control groupincreased by16.67%~16.78%, P<0.05, the difference is very significant; growth rateincreased by3.91%~4.5%,P<0.05,the difference is very significant; fatness increased by11.89%to11.99%, P<0.05, a significant difference, which suggesting that sandnolife MIC-Fplays a significant role in promoting the growth of turbot. Survival rate of the experimentalgroup than the control group increased by4.33%to5.33%, P<0.05, the difference issignificant.4. The water quality parameters in the following changes after feeding sandnolife MIC-F inturbot farming process: pH variation of the experimental group and control group is P=1>0.05, the difference is not significant; DO value of the test group and control group is P=0.356>0.05,the difference is not significant; temperature variation of the experimental groupand control group is P=0.365>0.05, the difference is not significant; the experimentalgroup and control group of ammonia nitrogen values change is P=0.553>0.05,thedifference is not significant; test group and control group of subchanges in nitrate values is P=0.15>0.05, the difference is not significant. This study illustrated the products ofSanolifeMIC-F which causes a insignificant variation in pH, DO, temperature, ammonia,nitrite.It is probably because the concentration of Bacillus don’t achieve the decompositionof the concentration of pollutants.
Keywords/Search Tags:SanolifeMIC-F, Cynoglossus semiloevis Günther, Scophthalmus maximus, growth, water quality factor
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