Effect Of Sodium Acetate And Sodium Butyrate On Expression Of Genes Related To Milk Fat Synthesis Of Dairy Cow Mammary Epithelial Cells And Acinus

The mammary gland of the lactating process important function gene expression changes is regulation of mammary gland growth, development, differentiation and milk synthesis, secretion, transport and other important physiological process based. Therefore, the important function of genes in mammary gland development to improve the genetic performance of the cows, promote the development of China’s dairy industry has a positive effect. Fatty acid synthase (Fas) is a multifunctional composite enzyme, is synthesized in vivo fat pathway of a key enzyme. Acetyl-coenzyme A carboxylase (Acc) is the rate-limiting enzyme of the fatty acid synthesis pathway, It catalyzes the first step reaction of the synthesis of fatty acids (FA), In the liver, fat and breast tissue, Acc significantly influenced by phosphorylation or dephosphorylation regulation, Also affected by the impact of the FA allosteric mechanism. DGAT1is the rate-limiting enzyme in the process of the TG synthesis, Associated with the dairy milk fat content. Purpose of this study aims to effect of milk fat precursor on milk fat synthesis gene expression, reveal the mechanism of gene regulation of cow milk fat synthesis. In the use of in vitro methods to obtain mammary acinar model, become a kind of effective simplified mammary development and lactation function research process basic method and important means.This experiment with mammary epithelial cells and in vitro dairy cow mammary acinar as research object, Using fluorescence quantitative RT-PCR method, has discussed the extraneous source increase sodium acetate and the sodium butyrate, effect of the key enzyme in the synthesis of milk fatty acids in mammary epithelial cells and mammary acinar model of Fas, Acc, Dgatl expression, the use of semi automatic biochemical analyzer by adding various nutrients in culture system of glycerin three greases content change, determined in vitro regulation of milk fat biosynthesis of sodium acetate concentration of16mmol/L; sodium butyrate concentration of0.75mmol/L; By transfection of Srebp gene silencing test, the results displayed in two-dimensional cell system and three-dimensional acinar system, disturbs Srebp to present the inhibitory action to3kind of important enzyme expressions. This study for further research on the regulation of lactation genes provides a theoretical basis.