2010～2011Analysis Of Molecular Epidemiology And Isolation And Identification Of Prrsv In Some Provinces Of China

Porcine reproductive and respiratory syndrome (PRRS) caused by porcine reproductive and respiratory syndrome virus (PRRSV) is a high mortality of infectious diseases characterized by pregnant sow reproductive failure and piglet respiratory disease. The prevalence of the disease have brought a huge loss to the global pig industry, especially after the2006epidemic of highly pathogenic PRRSV caused great difficulties due to its large degree of variation, to the research and development of efficient vaccines. PRRSV is a nested virus head, a member of the arteritis virus family, the genome length of approximately15kb and contains nine open reading frame (open reading frame, ORF).In this study, the1375copies of the10provinces in2010and1707copies of the12provinces in2011pig slaughterhouse and feedlot tissue samples were tested, and the positive rates were28.7%and16.75%. Detection analysis found that the positive rate of2011samples has declined compared to2010, testing for the positive samples most of the variable strains of PRRSV. Through the analysis of PRRSV variations in the prevalence of the disease in some provinces and cities in China, explore PRRS in the space group between the time of distribution and infection status; have a preliminary understanding of PRRSV genetic variation characteristics of popular rule.The application Mcar-145cells isolated from15of PRRSV by electron microscopy, indirect immunofluorescence assay (IFA) and TCID50determination to further determine the success of isolated of PRRSV. Downloaded on the NCBI standard sequence design primers to amplify ORF5full-length is603bp, the homology between isolates was98.8%～99.9%, nucleotide deduced amino acid sequence95.5%～99.5%.15PRRSV isolates and the American type were highly homologous,so they were inferred to belong to the Americas.9of the ORF5(151) of amino acid variation of HB-1,R151→K.151, the remaining six the ORF5amino acid (151) point and JAX1of the same, the ORF5(151) for R Phylogenetic tree, BFLN3-2010BFSD1-2011, and BFAH2-20113strains CH-la in the same branch, the second subgroup, other BFLN2-2.01thousand, etc.12with JAX1in the same branch, belonging to the fourth sub-group.Whole genome sequencing of the BFLN2-2010gene is15320bp. The Nsp2gene length are 2850bp encoding950amino acids, its Nsp2gene deduced amino acid two nucleotide deletion, a482in Nsp2protein has an amino acid missing, the other took place in the continuous absence of29amino acids between533and561, a total of30amino acids missing, with2006after the separation of highly pathogenic PRRS JXA1strains HUB1lines have a similar lack of nucleotide sequence characteristics. The homology of BFLN2-2010isolates and LV European standard strains was61.4%, compare to the American type standard strain VR-2332, the homology was89.1%, indicating that BFLN2-2010isolates belong to American type strains. Gene phylogenetic tree show that the distance between BFLN2-2010strains and CH-1a, the VR-2332, BJ-4genetic is farther, and BFLN2-2010strains and JAX1belong to the same subgroup.