Comparative Analysis Of Lws Gene For Sequences And Evolution In Lutjanid Fishes

The genus of Lutjanus fish attached to the Perciformes, Lutjanidae, is widelydistributed in tropical and subtropical reef fish and economically important fish in theSouth China Sea. This study employ phylogenetic and gene structure to analyse thesnapper in the evolution and structure of relations, The study selected mitochondrialfragments (COI) as a neutral marker and functional nuclear genes (LWS) tocomparative analysis for the evolutionary relationships and spciation by the LWSgene sequence analysis of its structure to further understand.In this study we selected14snapper samples wchich sampled from South ChinaSea, and it is very necessary to use DNA barcoding identification and screening outthe typical specimens of each species. The study also selected19species as aoutgroup (including vertebrate and invertebrate)together with the amplification of14snappers’LWS gene to analyse the phylogenetic relationship, The data show thatinvertebrate and terrestrial animals gather one branch respectively, and the amphibianis between terrestrial and fishes. In Lutjanus fishes of LWS compare with neutralmarker the result reveal that L. vitta and L. ophuysenii, L. kasmira and L. bengalensis,and L. malabaricus, L. erythropterus and L. sebae get the closely relationship. It canbe seen from the phylogenetic tree that blue snapper (L. kasmira and L. bengalensis)and red snapper (L. malabaricus, L. erythropterus and L. sebae) converged togetherrespectively, which associated with the obvious external morphological characteristicsof the body color, side banding pattern in the morphological classification system. Inhigh level phylogeny, the amino acid sequence alignment analysis show theevolutionary relationships is consistent with morphological classification: Lutjanus、Cypriniformes (zebrafish, goldfish) and terrestrial animals (human, rats and lizards)converged respectively. Comparison to the neutral mark of the phylogenetic tree, theL. vitta and L. ophuysenii occurred separation, the site of180in Amino acids theL.vitta is A but the L. ophuysenii show S. The above evidence suggest that in Lutjanusoverlapping area(uncomplete geographical isolation)of the fish ecology of the speciesduring the spciation the selective evolution of the opsin LWS gene may play animportant role. This study determined Lutjanus erythopterus and Lutjanus johni’s partial cDNAsequence and its3’UTR sequence respectively. Lutjanus erythopterus containsreadable coding frame693bp, encoding230amino acids, and the Lutjanus johnicontains readable coded frame690bp, encoding229amino acids; also the3’UTR ofLutjanus erythopterus is176bp and Lutjanus johni is187bp; analysis of these twosnappers LWS gene sequence, an AT-AC structure of intron containing in fragments.And the homology of alignment between Lutjanus erythopterus、Lutjanus johni andterrestrial vertebrate show that the snapper is highly homologous with bony fishes.