The Classification And Identification Of Tomato Phloem Protein Induced By Root-knot Nematodes

Tomato occupies an important position in our vegetable production. But a long time, tomato production has been severely affected by the disease. Root-knot nematodes diseases is one of the most serious diseases in tomato production currently, and it results huge economic losses to tomato cultivation, production and processing industry. In recent years, many studies found that jasmonic acid involved and played an important role in the induced resistance of plant. Different jasmonic acid mutants of tomatoes were used after the infection of root-knot nematodes with three stages:6h,24h and72h. The differentially expressed proteins were separated by two-dimensional gel electrophoresis. Finally,74differentially expressed proteins were found. Among them,24not express protein in jasmonic acid synthesis missing mutants(spr2) and50more than twice as much as expression in35S::PS.30well-resolved spots were identified by peptide mass fingerprinting (PMF) via matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). After database searching and bioinformatics analysis, results pointed out the expression level of proteins related to energy conversion, signal transduction, polysaccharide metabolism and cytoskeleton were totally difference. Proteasome subunit beta type-1, minor allergen hazelnut profilin and two hypothetical protein were not expressed in jasmonic acid synthesis missing matants(spr2). PSII related proteins, RNA-binding protein, formic acid dehydrogenase, fructose kinase-2and systemin flags precursor in jasmonic acid excessive express mutant were obviously higher than those in wild type tomatoes (WT) and jasmonic acid synthesis missing mutants(spr2). Among them, the identification of systemin flags precursor was powerful proved the role of jasmonate on the response of tomato to root-knot nematodes.