| Embryo implantation is the key factor to determine the successfulpregnancy in normal reproductive development process. The implantationof the embryo is a complex physiological process, it depends on the ofendometrial receptivity state and synchronous development of invasiveembryo. The changes in endometrial receptivity play a key role in theprocess of embryo implantation. Among these, the estrogen receptor(ER)and the HOXA-10gene play an important role in the regulation ofendometrial receptivity aspects. Estrogen regulate the endometrialreceptivity through the estrogen receptor, then, HOXA-10gene plays animportant role in the reproductive&embryo implantation anddevelopment aspects.Compared with natural pregnancy, there are a variety of factors inthe process of in vitro fertilization-embryo transfer(IVF-ET). then,endometrial receptivity is regulated by a variety of cytokines, proteinmolecules and genes in the formation process, and these specific regulatory factors present more significant temporal and spatialexpression characteristics in the process of development anddifferentiation of endometrial, have an important role in the formation ofendometrial receptivity.In order to further improve the success rate of the animal in vitrofertilization-embryo transfer pregnancy, from the ER distribution pointof view, this study used immunohistochemical method to detectexpression pattern that the different conditions treated mice estrogenreceptor expression in the endometrium of the estrous cycle in mice, toinvestigate the expression of the different periods ER receptordistribution in mouse endometrium whether there are differences,combined with the corresponding distribution of ER and embryoimplantation in the uterine implantation of the implantation window isconsistent, probe into ER regulation of mouse uterine implantationwindow and endometrial receptivity change. At the same time, the use ofRT-qPCR were used to detect the mice in each group under differentprocessing conditions endometrial HOXA-10gene expression, observedthe effect of HOXA-10gene expression in artificial injectionsuperovulation hormone and whether embryos being and other factors,and thus explore these factors on the role of endometrial receptivity.To investigate the effect of estrogen exerts on endometrialreceptivity, make active intervention on endometrial receptivity be possible, and improve the pregnancy rate of IVF-ET expression profile ofER in endometrium during estrous cycle of Kunming mice was assessedbased on immunohistochemistry.36female mice of the same age,depending on the approach, were randomly divided into three groups:natural estrous pregnant group, natural estrous pseudopregnantgroup(control group), and ovarian stimulation group. Three groups ofmice on day2,4,6and8, respectively, The removal of the uterinesamples, using immunohistochemical method to locate andsemi-quantitative research the expression of the different treatmentgroups of mice endometrial estrogen receptor quantitative research in thedifferent periods, to observe the distribution of ER changes in mouseendometrium.The immunohistochemistry results indicated, that no significantdifference of ER expression were observed in mice among natural estrouspregnant group, natural estrous pseudopregnant group, and ovarianstimulation group on day2(P＞0.05). While, on day4,6and8, theexpression level of ER in ovarian stimulation group was remarkablyhigher than that in the other two groups(P＜0.05). In addition, on day4and6, the expression level of ER in natural estrous pregnant group wasalso much higher than that of pseudopregnant group(P＜0.05), while onday8, no significant difference of ER positive rate was observed betweenthese two groups. Compared with the mice in pregnant group, endometrial receptivity of the other two groups was declined and thewindow phrase was delayed, however, no significant changes ofendometrial receptivity and window phrase between pseudopregnantgroup and ovarian stimulation group were observed (P＞0.05).By measuring different mouse uterus HOXA-10gene expressionlevels in natural estrous pregnant group and ovarian stimulation group, toinvestigate the factors such as the artificial injection superovulationhormones and whether the embryos exist to HOXA-10gene expression.The growth and development of better uniformity and consistency ofexperimental mice were randomly divided into three groups: naturalestrous pregnant group, natural estrous pseudopregnant group(controlgroup), and ovarian stimulation group. Three groups of mice on day2,4,6and8, respectively, The removal of the uterine samples, using SYBRGreen I RT-qPCR method, respectively, to detect HOXA-10geneexpression changes in the amount for mouse endometrium of three groupsunder different processing conditions, and comparative analysis betweenthe two groups.The RT-qPCR results indicated, HOXA-10gene expression can bedetected in the mice of ovarian stimulation group and natural estrouspregnant group endometrium, but there were differences in the time andquantity of the expression. Two groups of mice in group A and group C,the endometrium HOXA-10gene expression were tested positive in the estrous cycle, and expression intensity showed an increasing trend frompregnancy after the first two days, a gradual decrease to six days later;Compared with group B, group A and group C mouse endometriumHOXA-10gene expression intensity has increased, but the increase is notthe same. The expression level of group A has been higher in2-6daysafter the pregnancy, and its reach peak expression is higher than the peakof the expression of the group C. group A and group C HOXA-10geneexpression pattern in the endometrium, basic consistent with the mouseuterus during the implantation window period. |
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