Changes Of Reproductive Hormones Concentrations During Estrus Cycle And Early Pregnancy Diagnosis In Beef Cattle

Beef cattle industry should not only an excellent introduction of foreign species on thelocal beef cattle crossbreeding, but also rely on the excellent local cattle breed with localvarieties of beef cattle breeds ethnic characteristics. Quality characteristics of beef cattlebreeding domestic livestock as a rising star in China, in beef cattle production has played anincreasingly important role. Since1985, the Chinese Red Steppe after incubation, with its hotclimate of the endurance of a strong cold, disease resistance, the incidence rate is low, mellowquality of milk, fresh meat, meat is delicious, nutritious, good quality and well-known andexported to the sea internal and external, and by the China Green Food Development Centercertified as green food, known as "prairie pride." Yanbian cattle breeding cattle is one of fiveareas have been included in the list of species conservation. Yanbian Korean Korean beef cattleand the same clan ancestors, tall size, physique and firm, cold, rough feeding tolerance, diseaseresistance, reproduction and strong, easily fattening, meat production is high, Yanbian cattlemuscle cross-sectional shape was marbled beef unsaturated fatty acid content in the extremelyrich, delicate taste, unique flavor, by consumers, Yanbian beef quality was significantly betterthan with the other use either type of local cattle with the genetic basis of high-grade beef.In practical farming, mismatch, mismatch, early embryonic death (abortion), and repeatwith infertility and other reasons, can cause pregnant cows extension of the space, thus reducingthe reproduction rate of cows, is not conducive to the development of cattle industry. Estrustimely manner not only the identification and diagnosis of early pregnancy reduces the dams ofthe empty cup, reduce the dams calving interval and increase the reproduction rate of dams, andcan reduce the human, material and financial resources to waste, need to highlight thepropagation of new technologies in cattle breeding farms in the key role.The topics to Jilin province in the Simmental, Red Bull Yanbian cattle and grassland as theresearch object, the method by radioimmunoassay RIA detected estrus cycle of cattle blood andurine samples in the follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol alcohol (E2), progesterone (P4) and other hormone levels, estrous cycle to establish the curve ofthe reproductive hormones. Also developed to detect cows in heat meat identification anddiagnosis of pregnancy, progesterone colloidal gold test strip. To meet our beef cattle farmon-site testing and screening test needs to provide for the production of some theoretical basisand practical guidance. The main results are as follows:1. Jilin meat cow estrus cycle changes of reproductive hormones in vivo studiesThe system of Jilin region Simmental, Red Bull Yanbian cattle and grassland as theresearch object, the method by radioimmunoassay RIA system detects the estrous cycle of cattleblood and urine samples in the follicle stimulating hormone (FSH), luteinizing hormone (LH),estradiol (E2), progesterone (P4) and other hormone levels, estrous cycle to establish the curve ofthe reproductive hormone, FSH among the three species is not pregnant and had no significantdifference between pregnant (P>0.05); three LH in21d varieties were higher than had beenpregnant and not pregnant, significant difference (P <0.05); three species have been heat contentof the first21d P4were higher than those not pregnant pregnant, the difference was significant(P <0.01); pregnant cows first21d progesterone were significantly different, the three localvarieties by testing cattle identification and progesterone on the estrus diagnosis of earlypregnancy is feasible, the specific criteria of the ":1) Red Steppe, Simmental estrus21daysserum progesterone values greater than2.5ng/mL to determine is pregnant, Yanbian cattleestrus21days to determine serum progesterone greater than4.0ng/mL been pregnant.2), RedSteppe, Simmental day estrus progesterone in serum less than1.0ng/mL determine heat, heat theday of Yanbian cattle less than2.0ng/mL determine serum progesterone estrus.2. Estrus and early pregnancy diagnosis identification strip of colloidal goldUse of beef cattle and non-pregnant cows pregnant the first difference of progesterone21d, to develop detection and identification of beef cow estrus progesterone pregnancy diagnosiscolloidal gold test strip. To meet our beef cattle farm on-site testing and screening test needs toprovide for the production of some theoretical basis and practical guidance.