Activity Separation Of One Antagonistic Actinomycetes Of Orchid Soft Rot

The materials were migratory marine fishes, which were collected from Pingtan intertidal zone of Fujian province, tropical freshwater fish in Fuzhou Taijiang flower market.18Fungi were isolated from fresh marine fishes.14Bacteria and27fungi were isolated from fresh freshwater fish. There were21marine bacteria and25marine actinomycetes, which were kept in our laboratory.266Endophytic fungi were isolated from Ginkgo biloba in laboratory, which had been screened antibacterial activity.From antibacterial experiment of soft rot bacteria and screening antitumor activity, screening one marine actinomycete and another fungus from tropical freshwater fish. Both actinomycete and fungus whose metabolites had steady activity, and would not degrade by subculture.Disease tissues of Cymbidium spp., which as experiment materials. NGM was fermentation and selection medium. One pathogenic bacterium was got. Through the experiment of bacterial physiology, biochemistry and Koch Rule, this was Erwinia carotovora. Actinomycete strain0214had good antibacterial activity by the Oxford cup and Filter paper diffuseion methods. Test of stability and optimization of culture, which still had stability of the antibacterial activity.In addition, the antitumor activity was screened with the human hepatoma cell line BEL-7404by MTT method; the result showed that fungus MQ-3had good antitumor activity. According to morphology and cultivation characteristics of MQ-3, it was identified as Aspergillus sp.. It provided materials for further separation active compounds of antitumor.