Study On The Improvement Of Disease Advances Resistant Of Maize Inbred Line Jing24with Molecular Marker-assisted Selection

Serious damage caused for corn production Southern Corn Rust and Stalk Rot of corn happening of the pop, the use of molecular markers to domestic backbone for improved breeding excellent inbred lines disease resistance, resistance to the southern rust resistance and established corrosion of the disease stems nils, to discover and use of r gene resources, screening and foster resistant varieties, control the spread of the disease is an effective way. In this experiment based on the former research, the use of corn to the south and the stem rust corrosion resistant breeding excellent inbred lines of expression of disease’s Qi319and1145for donor parents, Jing24for receptor parents, through the hybrid, back to the intersection of means, constructed respectively in Jing24for genetic background respectively rust corrosion resistance to the south and the stem of the disease nils, and by using the molecular marker assisted selection method, the parents were in between the prospect of high polymorphism primer and background of primers, combining the rust and corrosion resistance in the field of the disease stems phenotype appraisal, won the homozygous resistance and corrosion resistance to stem rust south of the Jing24improved disease. Will the Jing24(rust resistance to the south) and Jing24(resistance to stem rot disease) among the hybrid yield per plant, get the polymerization two resistance genes breeding excellent inbred lines of the group. Using fluorescent markers and multiple primer composite electrophoresis technology, combining obviously improving the detection efficiency, to make the result more accurate and reliable, to use the lower costs to test the effect of an ideal choice for purpose, and compared the polyacrylamide gel electrophoresis, improved the efficiency of molecular markers background selection, for high throughput of SSR markers detection platform research and development has laid a foundation.Results:from14to prospect of primers were ultimately with the southern rust resistance gene heredity distance is nearly, amplification stability, which located in resistance genes upstream and downstream two primer Phil18and P2, used to target traits of the future of the choice.460per generation of indoor BC2molecular markers detection results and hainan nature of disease prospect is proved the validity of the selection markers. Can see, like the south for this quality of rust character, the use of molecular markers to target traits carries on the prospect of choice is effective, double marking choice than single mark obviously accuracy choice to high, and from the field of natural conditions and the limits of the artificial inoculation technology restriction. Use in Qi319and Jing24together with obvious difference between42primers backcross group background choice, has got the background screening in the response rate of more than98%pure and disease resistance of the Jing24improvement.From10to prospect of primers were in248and337the final two primer for stem rot disease resistance genes of molecular marker assisted selection. Research shows that carry the effect of plant resistance genes than not take effect of plant disease resistance Lord QTL obviously improve the rate of36%, two molecular markers were the results of the testing similar, so use only a can achieve a goal, save resources. In1145and the use of the difference between Jing24has obvious49primer for back into groups of background choice, has got the background screening in the response rate of more than97%pure and disease resistance of the Jing24improvement.This research use has obtained in Jing24for genetic background respectively and the stem rust corrosion resistance to the south of the disease homozygous improved breeding excellent inbred lines as parents, in BC3F3generation hybrid of two parents, in order to achieve the purpose of r gene polymerization breeding, has obtained the F1hybrid material.