Genetic Diversity Study Of DH Lines In Chinese Cabbage

The Native-PAGE was optimized, and on this basis, the268plants ofDH lines in Chinese cabbage (three DH lines in Chinese cabbage and125DHmaterial of DH lines in Chinese cabbage) were marked by SSR molecular markers，and labeled datas were constructed clustering figure of three DH lines, as well as125plants of DH population. The main results are as follows:1. Many factors including gel’s polymerization rate and fragments’ migrationratio affected detection effect of native Polyacrylamide Gel Electrophoresis(Native-PAGE). The gel’s polymerization rate, the detection effect of PCR productsby two kinds of gel stock solution (40%Acr-Bis (19:1) and30%Acr-Bis (29:1)prepared in four concentrations (5%,7%,9%,11%) were studied in this paper. Theresults showed that the gel’s polymerization rate is accelerated with increasingtemperature in the range of4-30℃; APS remains the same at different temperaturesand TEMED:APS suitable ratio is different:4℃(winter), ratio is1:10,25℃,30℃(spring and Autumn)(summer),1:20are better. Detection of the effect was differentin gel by two different stock solution prepared same gel concentrations,30%Acr-Bis(29:1) is better than40%Acr-Bis (19:1). And DNA fragment separation is good, withclear band type, high resolution in the gel that was prepared7%by former stocksolution.2. Used4representive cultivars of DH lines in Chinese cabbage，at last, total of12pair primers being polymorphic and informative selected out of122SSR markers.and the application of the12pairs polymorphism primers, all DH lines in Chinesecabbage of DNA were extendincreased by PCR, and amplification products wereDetected by30%Acri: Birs (29:1)7%Native-PAGE. The observation result ofdetection that efficiency of amplification was different in different DH lines inChinese cabbage by SSR primers. A total of37bands were amplified with29bands ofpolymorphic for DH lines in Chinese cabbage Y360(60), and the polymorphism ratewas78%. DH line in Chinese cabbage Y531(48) amplified a total of43bands with24polymorphic bands, the polymorphism rate was55.8%; A total of41bands were amplified with36polymorphic bands for Y531(44), polymorphism rate was87.8%;125DH populations in Chinese cabbage was expanded61bands with53polymorphicbands, the polymorphism rate was86.9%.3. Y360DH lines in Chinese cabbage were divided to the nine groups at thegenetic distance of0.43, and the genetic distance range is0-0.71.YF05DH lines inChinese cabbage were divided to the two groups at the genetic distance of0.752, thegenetic distance in the range of0.08-0.81. The materials of first group of are hairy ofleaves, but second species leaves are light. Y531DH lines in Chinese cabbage weredivided to the nine groups in the genetic distance of0.41, the genetic distance in therange of0-0.77. Most of the material was distributed in the upper part, only a fewmaterials scattered in the upper and lower part in the cluster map.4.125DH populations in Chinese cabbage were divided to the seven groups atthe genetic distance of0.69, the genetic distance in the range of0-0.79. Materials ofall groups there is no clear boundaries on the phenotype.