Cloning Of Tolerance-related Genes During Cotton Pollen Developmental Stages Under High Temperature Stress

In this study, a forward cDNA-SSH library and a reverse cDNA-SSH library were established by suppression subtractive hybridization using pollen of HLY11(H) and TS18(T),a heat-tolerant cotton and a heat-sensitive(Gossypium hirsuutm L.) were conventional strain under high temperature stress.The library reorganization rate of95%to be verified by PCR.We randomly selected400positive clones for sequencing and analysising.Totally342available EST sequences and278non-redundant unigenes were acquired by splitting and clustering analyses of the cDNA sequences. The results of BlastN showed that211unigenes had homologous sequences in GeneBank database,among which164unigenes had significant protein homologies and47unigenes were unknown or putative proteins,while the other67unigenes had no protein homologies.152unigenes were mapped to46metabolic pathways by KEGG analyses,in which there were14pathways containing31unigenes at P-value less than0.05. A large number of high-temperature-stress-responsive genes involved in heat shock protein,antioxidant enzymes,carbohydrate metabolism,transcriptional factor,trans-membrane protein and so on were found from the cDNA libraries,and the investigation suggested that the starch and sucrose metabolism,glutathione metabolism,planr-pathogen interaction and oxidative phosphorylation may closely relate with tolerance to high temperature stress in cotton anther.