The Preliminary Study On The Drought Tolerance Of Transgenic ArCIPK23Potato

The transgenic AtCIPK23potato lines have been obtained by Agrobacterium-mediated genetic transformation methods, and PCR,Southern blot, RT-PCR analysis have identified the AtCIPK23gene was successfully integrated into the potato genome, and it is expressed in transcription level, and the low-K+stress tolerance of these transgenic potao can stronger than non-transgenic potato.The phenotype research before showed that maybe the drought tolerance of transgenic AtCIPK23potatos stronger than non-transgenic potato. In order to know the truth, this study use transgenic lines to research. PCR analysis have shown that after repeatedly propagation in vitro, AtCIPK23gene still exist in the experimental lines L1, L3, L4, L5, L6, L7, L8, L10, L11, L16, L17, L18, L20, L23. L25, L26, L28, L30, L31, L34, L36, L38, L40, L41. And then analysis on difference for drought responses of potato lines in vitro by observing the morphological responses under PEG6000simulation of drought stress conditions.Studies have shown that all of lines can be divided into four categories, L8, L10, L16, L20, L25, L30, L31, L38for the first class, L5, L6, L7, L11, L17, L18, L23for the second class, L1, L26, L34, L36, L41for the third class, W (wild type), L3, L4, L28, L40for the fourth class, and the strength of drought tolerance is:class one>class two>class three>class four.Based on the results above, the study choose L18(class two), L23(class two), L25(class one), L34(class three), L38(class one) and W(class four) for the further physiological and biochemical research. The results show that transgenic lines can significantly reduce the loss rate of excised-leaf water, under the PEG simulated drought stress conditions, transgenic lines can maintain higher water content by increase the content of osmotic adjustment substances such as soluble sugar and proline, and have the higher antioxidant enzymes activity such as POD and SOD, the damage of these transgenic lines are less than non-transgenic plant, and with a lower content of MDA under the drought stress. All results above demonstrate transform AtCIPK23gene can increase the drought tolerance of plants.