Spatial-temporal Dynamics And Security Appraisal Of Btcrylac Toxin Protein In Poplar-cotton Composite System

With the large scale popularization and cultivation of transgenic Bt plants year by year, it creates great social and economic efficiencies. But people pay more attention to the safety of the release of transgenic plants. Eco-safety of transgenic plants means directly or indirectly impinge upon ecosystem including the safty of non-target organism, resistance of the arget pest to transgenic plants, the population growth of secondary pest, gene drift and exotic weeds and so on. So it is a very important issue of the influence of transgenic plants on the ecosystem.From2009to2011, we tested exogenous BtCrylAc toxin protien spatial-temporal dynamics from different tissues of transgenic poplar and cotton at development stage and discovered the distribution, enrichmentde and gradation of BtCrylAc toxin protien in soil. It provide scientific basis for transgenic plant risk assessment and security appraisal of soil ecosystem.poplar741, chinese white poplar, poplar Pb29, transgenic Guoshen GK45and non-transgenic cotton Xinzaolu36were constructed poplar-cotton composite system. Useing ELS A technology, we tested exogenous BtCrylAc toxin protien spatial-temporal dynamics from different tissues of transgenic poplar and cotton at development stage.The poplar-cotton composite system was practicable in our research. The results showed that the contents of exogenous BtCrylAc toxin protein branch leaves, short branch leaves and root firstly increased and then decreased in the growth and development of transgenic poplar Pb29. But the contents of exogenous BtCrylAc toxin protein had not been accumulated year by year in poplar Pb29. It was showed no strong correlation between the contents and year. The contents of exogenous BtCry1Ac toxin protein, stems and leaves decreased along with the growth of transgenic Guoshen GK45. The contents of exogenous BtCrylAc toxin protein of stems decreased more slowly and always maintained a high level, while that of roots and leaves decreased rapidly and reached a minimum level to the late plant growth and development, were less than that of stems. The contents of exogenous BtCrylAc toxin protein had not been accumulated year by year in cotton. There was no change regularity between the contents of exogenous BtCrylAc toxin protein and the three years.Although it was tested BtCrylAc toxin protein in topsoil of non-transgenic and transgenic poplar and cotton composite system by means of ELSA technology. There was no significant difference in topsoil of poplar-cotton composite system.It was studied that the stable expression of exogenous BtCrylAc gene of transgenic poplar Pb29and transgenic cotton Guoshen GK45in poplar-cotton composite system. This further proved the expression of Bt gene was related to plant development. The contents of exogenous BtCrylAc toxin protein in transgenic Guoshen GK45was significant higher in transgenic poplar Pb29. Few of BtCrylAc toxin protein in topsoil of non-transgenic and transgenic poplar and cotton composite system has been detected. But there was no change regularity between the contents of exogenous BtCrylAc toxin protein and the three years.