Genetic Diversity Of Eight Rabbit Populations Based On STR Loci Analysis

Genetic diversities of Suxi Angora rabbit, Wanxi Angora rabbit, Rex rabbit, New Zealand white rabbit, Fujian yellow rabbit, Min xi’nan black rabbit, Jiuyi mountain rabbit and Belgian rabbit were studied using18microsatellite markers. This research combined the technique of PCR and the fluorescent automated detection, as a platform on an ABI3730x1genetic analyzer (Applied Biosystems) and GeneScan-500as size standard. Alleles were scored using GeneMapper v4.0software (Applied Biosystems).The genetic diversity was disclosed by calculating the allele frequency, heterozygosity (He), effective number of alleles, polymorphism information content (PIC) and Reynolds’genetic distance. Phylogenetic Relationship was analyzed by the neighbor joining method (NJ). The genetic variability within populations and genetic differentiation among populations were estimated. Our main results were summarized as follows:1. The8rabbit breeds with abundant genetic resources and have a large range of variation. The overall expected heterozygosity of all populations and PIC of all loci were0.614and0.583respectively. The average PIC of all loci were from0.273to0.709.2. A total of142alleles were detected in8populations with18microsatellite markers. Microsatellite loci have alleles from4to13, and the average number of observed alleles was7.889±0.137. The effective allele number was3.086±0.072, the smallest was1.459in loci6L7C11, and the largest was5.108in loci D3Utr2.3. Reynolds’ distance values varied from0.103(between Suxi Angora rabbit and Wanxi Angora rabbit) to0.351(between Suxi Angora rabbit and Min xi’nan black rabbit). The phylogenetic relationship based on DR was analyzed, an unrooted consensus tree was constructed, and the8rabbit populations were divided them into3groups. The first group contained Suxi Angora rabbit, Wanxi Angora rabbit, American Strain Rex rabbit, Jiuyi mountain rabbit and Min xi’nan black rabbit; New Zealand white rabbit and Fujian yellow rabbit comprised a second group; Belgian rabbit was the third group.4. Compared on four rabbit groups’(New Zealand white rabbit、Fujian yellow rabbit、 NxF rabbit and F×N rabbit) slaughter performance and two hybrid progenies showed heterosis. To the NxF rabbit and FxN rabbit the heterosis rate and3.68%and8.14%separately, indicated that the FRN rabbit’s heterosis was more obvious.The validation of results in detection of polymorphism were also showed the same result.5. The genetic distances of New Zealand White Rabbit, Fujian yellow rabbit and their filial generations revealed that F×N rabbit compared smaller genetic distance than NxF rabbit with two parents. The F×N rabbit was closer to the genetic traits of both parents. And the Slaughter performance showed the same results.