Control Of Nilaparvata Lugens By RNA Interference

RNA interference (RNAi) is the specific downregulation of gene expression by endogenous or exogenous double-stranded RNA (dsRNA), which is cleaved into siRNAs with the size of21-25nt by dsRNA-specific endonucleases referred to as Dicers. SiRNA is assembled into an RNA-induced silencing complex (RISC) in conjunction with the Argonaute protein, giving the rise to the degradation of a homologous target mRNA specifically. The potential of using RNAi effects to protect plants against insects by downregulating essential gene functions in the herbivore, thus resulting in its death, has been recognized since the discovery of RNAi. In addition, the method of RNAi would be effective, safty and more durable, which gives it preferential right in protecting insects.The key to the application of RNAi technology to give resistance to herbivorous insects is:(i) identification of a suitable and effective insect target;(ii) dsRNA delivery, which includes in planta expression of dsRNA and delivery of sufficient amounts of intact dsRNA for uptake by the insect. In this study, we select Cationic Amino Acid Transporter, Hexose Transporter1, Ribosomal protein L23and Ribosomal protein S3e as the target of dsRNA. Four RNAi vectors aimed to selected gene were successfully constructed and transferred into Oryza sativa mediated by Agrobacterium. Western Blot analysis indicated that glyphosate-resistant gene1174was expressed successfully in transgenic rice leaves. The expression of dsRNA in transgenic rice were detected by Northern Blot. But bioassays with Nilaparvata lugens demonstrated that these transgenic plants were not highly resistance to insects.