Cloning And Functional Analysis Of Glutenin In Triticum Aestivum

Wheat is one of the most important food crops in the world. It is an important source ofhuman energy and plant protein. Since always, the primary objective is high-yield anddisease-resistant wheat cultivating. High quality wheat is dependent on foreign imports. Inrecent years, along with the increasing living standards, people pay more attention to qualityand nutritional quality of wheat processing. Glutenin is one of the seed storage proteins, itpresent in the endosperm of wheat grain. Due to their characteristics, they exhibit featuresimportant for the technological properties of flour. Through genetic, breeding and transgenicstudies, it has been found that the expression of HMW-GS certain subunit is correlated withbread-making quality. Study of LMW glutenin gene function is less than that of HMWglutenin. Specific studies of these subunits in molecular breeding programs have contributedto the genetic improvement of breadmaking quality in wheat.In order to study the function of the low molecular weight glutenin gene, we contrastfifteen low-molecular-weight glutenin genes and found highly similar region betweendifferent genetics. Select high similarity fragments, designe primers, constructed RNAiinterference vector. Transformed it into the callus of wheat by particle gun and finally got2positive plants. Analyze its seeds by SDS-PAGE.The target gene is the one from Triticum monococcum named TmLMW i1. Thesequence analysis showed that the gene was1038bp, which encoding345amino acid,molecular weight of39.726kDa. It is a kind of LMW-i type glutenin subunits. Designeprimers. Constructed vector and transformed it into Triticum aestiuum199. Five positiveplants were got finally.We got eleven mutants lacking of HMW-GS subunit from Shi4185by EMSmutagenesis. There are five single subunit mutant types:1Ax1deletion,1Dx2deletion,1Bx7deletion,1By9deletion,1Dy12deletion. Furthermore, four mutants with deletion ofsubunit pair1Dx2+1Bx7,1Ax1+1By9,1Bx7+1Dy12,1Dx2+1Bx7+1By9. Two kinds ofmutants express other subunits,1Bx13+1By16and1By8. They missed1Ax1+1By9and1Ax1at the same time. In this study, eleven mutants with deletion of different high molecular weight glutenin subunit was got by EMS mutagenesis. This was rarely been reported. Thisuseful material can help us further identify the function of the high molecular weight gluteningene in the same genetic background, cultivate suitable wheat varieties for different purposes.Previous studies have shown that1Ax1and1By9were associated with poor bread-makingquality, except mutant10G112and10G123. These materials are useful for wheat qualitybreeding.