Fingerprinting Patterns And Linkage Analysis Of SSR Markers For Loquat(Eriobotrya Lindl) Germplasms

Loquat [Eriobotrya (Thunb) Lindl] is a subtropical evergreen fruit tree originatingin China that belongs to the Maloideae subfamily of the Rosaceae beside apple or pear.SSR sequences are the reproducible, stable, rich polymorphism DNA molecular markers.Fingerprint patterns of the SSR markers for33loquat varieties in national loquatgermplasm resource garden were constructed by specifically amplifying polymorphismfrom7pairs of primers screened from51pairs of primers of the relatives Malus andPyrus. The results showed that, the uses of seven primers, Hi01d05F, Hi12f04F,CH02B10F, Hi03a03, Hi03e04, Hi04a05and Hi02c07, could distinguish33loquatgermplasm from one another, completely, providing an essential criterion forclassification and identification of the loquat resources.An F1population consisting of70individuals,derived from the cross between’Bingtang’ and ’Guanyu’ cultivars, was used to analysis the relationship of them. A totalof55scorable simple sequence repeat (SSR) loci resulted from the25SSR primer pairsscreened from80SSR primers in the F1progeny. Comparing with the linkage mapconstructed by Gisbert found that9pairs of the same primers amplified19locis, ofwhich15sites were differences, χ-square test showed that the existence4linkage loci,Hi04a05-1000and Hi04a05-660,Hi15h12-700and Hi15h12-200.SSR marker has animportant application value for species classification,identification and geneticrelationship analysis of loquat germplasm resources.