Proteomic Research On Lung Tissue Of Mice Exposed To Ricin Aerosol

Aerosolology covers microbiology, nosetiology, aerodynamics and Lemology. The duty of Bio-aerosolology is to defense damage of aerosol for pathogenic microotganism and toxic etal and protect human health with air biology-related technology. Respiratory system as aerosol particles air sexually transmitted first a protective screen, is the key research areas aerosol discipline. The respiratory system consists of the lungs and the system of airways connecting the pulmonary parenchyma to the exterior environment, and is consequently, by its nature, open to the exterior environment. Toxic possesses advantages of mass production, long-term storage, wide targets,low dose, but disadvantages of injecting easily and protecting hard. It’s the gap of biological aerosol science and immunology.As is known to all. ricin is hot to toxicology research. Ricin as the most powerful plant toxin, taken as category Ⅱ bio-threat pathogens by U.C.CDC. Its mechanism of toxicity has been studied for years, but there are still many phenomena can not be explained by existing theories, a nd far fewer studies on aerosolized ricin, there is a biggap with foreign countries. We design the system of exposing aerosol of ricin to inject live mice, in order to abserve changes of mice’s respiratory and to research proteome of lung, And then using proteomics technical analysis ricin aerosol exposure mice, the difference of lung tissue protein expression, find out and screening and respiratory damage related proteins. Wilkins and his team put forward the concept of proteomics first, and2D is able to separate tissue and cell to the thousands of proteins in the most effective method nowdays. The experimental results show that by using the TSI3079ricin aerosol size stability distribution0.7-0.9μm. median in0.1μm, total concentration of aerosol0.2mg/m3. After exposure from pathology in mice that similar symptoms of acute respiratory distress syndrome, which led to the whole body organ failure and death. Using2D electrophoresis and MALDI-TOF-MS detect six different function key proteins:Respectively with the fat metabolism, protein expression regulation, REDOX, neurons synthesis, the function such as related elements in cancer. To build biological aerosol infection model and lung deposition model offers new experimental basis, for ricin aerosol particles cause respiratory system damage mechanism research laid a theoretical foundation, at the same time, is expected to further clarify the poison ricin to provide a new direction mechanism for respiratory system which drug development provides new targets and opportunity.