Strong Aroma Style Flue-cured Tobacco Soil Microbial Micro Ecological Environment

ABSTRACT This article based on studies of Moisture Soil, yellow cinnamonsoilbrown soil, Yellow-brown soil and meadow cinnamon soil in flue-cured tobaccogrowth period, collected rhizosphere and non-rhizosphere soil, studied Rhizosphereand non-rhizosphere the number of microorganisms, soil enzyme activity, soilmoisture content,and We inventoried The Bacteria community and the effects ofXuchang and Nanyang tobacco field，using16S PCR-DGGE and clone libraries tostudy the diversity of soil microbial community in flourishing-growth period.Themain conclusions are as follows:(1)The Soil of rhizosphere and non—rhizosphere contained more richmicroorganism resources and different types showed some variance. flue-curedtobacco rhizosphere and non-rhizosphere micro-organisms have shown the number ofthe same law: the rhizosphere soil microorganism quantity was higher than that ofthe non-rhizosphere soil；the number of bacteria were the most and far greater thansome other microorganisms.The bacteria，fungi， actinomycetes and potassiumbacteria total amount in rhizosphere and non—rhizosphere brown soil were highest.The Phosphate-solubilizing bacteria，living nitrogen fixing bacteria and ammonifiertotal amount in rhizosphere Moisture Soil were highest.(2)soil enzyme activity in the rhizospheric were higher than thenon-rhizosphere.Catalase activity and Urease activity have a single peak curve patternwith Maximum that occurred at different time in rhizosphere and non—rhizospheresoil Which Catalase activity at after plant out90days and Urease activity60days.The maximum values occurred in40-60d after transplanting in rhizosphere andnon—rhizosphere soil. The Catalase activity and Invertase activity in rhizospherebrown soil were highest. The Urease activity in rhizosphere Moisture Soil werehighest.(3)The contents of available nitrogen, ph,organic matter was along with theadvance of flue-cured tobacco growth period showed elevated–lower in the soil moisture content with Maximum that occurred in30-90d after transplanting.The soilmoisture content have shown for the rhizosphere>non-rhizosphere.(4)Correlation analysis indicated that there existed a close relationship betweenthe in Soil of microorganisms，soil moisture content and soil enzyme activity. Soilenzyme activity and soil bacteria, actinomycetes, fungi and other microorganisms hadsignificant correlation， and can be used as avaluable index． The Soil ofmicroorganisms were the main factors affecting the soil enzyme activity andwhich had the intensity of correlation of the bacterium and azotobacteria. soil organicmatter was significantly correlated with. soil enzyme activity. The correlation of TheCatalase activity,Invertase activity and soil moisture content was most significant.The interaction among Soil of microorganisms，soil moisture content and soil enzymeactivity stabilize the soil ecological system and plays a decisive role to make soil usedsustainable.(5) We inventoried The Bacteria community and the effects of Xiangxian andFangcheng tobacco field，using16S PCR-DGGE and clone libraries．DNA sequenceanalysis indicated that most of the PCR-amplified16S rDNA genes Similaritybetween94%-100%, owing to its high level of similarity and reliability.. the contrastresult belonged to the Proteobacteria and the next Bacteroidetes, Firmicutes andAcidobacteria. Phylotypes of Proteobacteria were the dominant group in both fieldwhich Bradyrhizobium and Sinorhizobium were the most dominant and Xiangxian tof a non-distinctive character willmigerl.the reason of the reducetion of ecosystemdiversity maybe related to tobacco field soil continuous cropping and paying noheed to ecological protection. At the same time, it may also be caused by diseasesand insect pests widespread occurrence, the degradation of the field was one of theimportant reasons.