Using Recombinant Rnbred Rines Rreate Rodging Rielding Sorghum Sudan The Grass Germplasm Research

Lodging is an important factor that affect the yield of Sorghum Hybrid Sudangrass. To further cultivate high yield, new lodging resistant varieties of Sorghum Hybrid Sudangrass, we first created the F9recombinant inbred lines118of Sorghum Hybrid Sudangrass(Sorghum314A x brown shell sudangrass). Then we measured plant height, ear length, stem fresh weight, stem weight, section II,1/2the height of the bending force, the center of gravity height, root weight and other agronomic traits and calculated the coefficient of its lodging. The correlation of the lodging coefficient and main agronomic traits and the SRAP molecular markers were analysised. The main results are summarized as follows:(1) We created the F9recombinant inbred lines of the Sorghum Hybrid Sudangrass based on descendant materials of314Ax brown shell sudangrass.(2) We screened three (11gz-1211gz-95and11gz-114), strong lodging resistance, high yield materials in118F9Sorghum recombinant inbred lines. We found that the bigger the lodging coefficient the greater Lodging area of the Sorghum Hybrid Sudangrass.Meanwhile, lodging coefficient was highly positive, correlation with plant height, stem fresh weight and stem weight,It was negative correlation with the root weight and breaking-resistant strength. The result indicate that lodging coefficient evaluation is feasible to evaluate lodging resistance of Sorghum Hybrid Sudangrass.(3) Through optimization of sudangrass SRAP-PCR reaction system factors to establish theoptimal reaction Concentration or content of the total systemy. The20μl total volume components are as follows:10*Buffer2.5ul, MgC122.5μl, DNTP2.5ul, primer2μl Tap enzyme0.15μl, template DNA1.Oμl, ddH2O9.35μl.(4) Research found that the results of analysis of agronomic characters are consistent with molecular marker analysis. We screened strong lodging resistance, high yield materials(11gz-12,11gz-95and11gz-114et al).And11gz-83is also a lodging resisrance material. The results will help us in regional testing and cultivar identification.