Studies On Newcastle Disease Oil Emulsion Inactivated Vaccine

This experiment, the Newcastle disease LaSota kinds of poison inoculated by the cavity of the chick embryo proliferation, and collection of allantoic fluid for the determination of the the its hemagglutination price, with10%Formalin inactivated preparation of the aqueous phase, with Sieppic (France) MONTANIDE the ISA206adjuvant (FCA) Newcastle disease oil emulsion vaccine. And Newcastle disease oil emulsion vaccine sterility test, physical properties testing, security testing, shelf life determination, and finally with the preparation of inactivated Newcastle disease oil emulsion vaccine and Indonesia Newcastle disease infectious bronchitis bivalent live vaccine immune28-day-old chicks and350-day-old laying hens. Different times after immunization blood hemagglutination inhibition test method for the determination of HI antibody titers. The immune effects of different day-old chicken Newcastle disease oil emulsion inactivated bivalent live vaccine, the new branch of the vaccine and Indonesia chicken.The results showed that Newcastle disease oil prepared water-in-oil emulsion inactivated vaccine the inspection.4℃refrigerator for6months after stratification stability. Newcastle disease oil emulsion inactivated vaccine, the HI antibody test that can produce7.05the log2of antibody28-day-old chicks immune7d;15d, the HI antibody titers up to9.531og2; to90d HI antibody5.951og2. After7days,350day-old laying hens immune antibody7.1the log2;45d HI antibody titers up to Il.llog290d after the antibody titer was8.9the log2. Compare Indonesia Newcastle disease Infectious bronchitis bivalent live vaccine produced HI antibody titers, and long duration.