Molecular Cytological Observation On Derivatives Of Common Wheat With Rye

Common wheat (Triticum aestivum L.,2n=6x=42, genome AABBDD) is one of the important food crops in the world. Rye (Secale cereale,2n=2x=14, genome RR) is an important alien species for genetic improvement of common wheat. In a previous study, we obtained some F8lines, derived from an interspecific hybrid plant between common wheat and rye. These lines showed good fertility and consistent characters in agronomy. In order to understand the chromosome behaviors of wheat-rye hybrids and to improve the efficience of chromosome transferring from rye into wheat, this study is aimed to characterize their chromosome constitutions of39lines. The main results are as follows:1. The39wheat-rye derived lines showed largely differences among plant height, tiller number, main spike length, spikelet number and grain weight. Since these lines were derived from a single F1hybrid of wheat with rye, these differences suggests some variations induced by wheat-rye wide hybridization.2. ZBY40has44chromosomes and the rest38lines have42chromosomes. At meiotic metaphase â… , most of the chromosomes are paired into bivalents, thus indicating that they are stable in cytology.3. Using rye total genomic DNA as a probe and Chinese Spring DNA for blocking, genomic in situ hybridization (GISH) analysis indicated that the nine lines ZBY35, ZBY38, ZBY40, ZBY41, ZBY47, ZBY48, ZBY56, ZBY58, and ZBY62had a pair of rye chromosomes. Based on the chromosomes number, it is suggested that ZBY40is an addition line and the rest eight liens were substitution lines. Except the nine lines, no rye chromosomes were detected in30out of the39lines. Further works on molecular studies of the30lines will help us to illustrate the mechanism of genetic variations induced by wide hybridization.