The Screening Of Lactococcus Lactis Ss Lactis From Fowls And Effects On The Performance Of Broiler By Feeding Of This Microcapsulated Probiotic

In this paper, the strain identified by BIOLOG automatic micro-analysis system and16S rRNA, was isolated from the bowel of healthy AA broilers. Pathogen inhibitory ability, growth performance, acid producing ability and the survival in the cruel environment also been studied. And180AA broilers were randomly assigned to three dietary treatments with three groups and6replicates with each group, feeding3different diets with the same recipe showed the effect of microcapsulated Lactococcus lactis on growth performance of AA broilers.Chapter I，Lactococcus lactis was isolated from the healthy AA broilers and it can inhibit E.coli K88, K99, Salmonella, Pasteurella and Staphyloccocus aureus Rosenbach, then identified by BIOLOG automatic micro-analysis system and16S rRNA, named it GJ00007which take a strong inhibition to E.coli K99.Chapter II, the Growth metabolic curve and the survival in the cruel environment which shows that GJ00007has the fast growth, the high L-lactic yield and the high tolerantion of reverse environment (including H2O2, artificial Gastric Juice and intestinal juice) were described,Chaper III,180AA broilers were randomly assigned to three dietary treatments with three groups and6replicates with each group, feeding microcapsule, the same concentration bacterium fluid and basic diet were fed as3different diets to the broilers.The result shows that feeding AA broilers with Microcapsulated GJ00007increased16.94%(P<0.05) and11.25%(P<0.05) in ADG, while16.13%(P<0.05) and6.18%in FCR, and increased the relative weight of thymus and bursa of6weeks old. These results indicated that microcapsulated GJ00007could increase the ADG and FCR of AA broilers and increased the relative weight of thymus and bursa of6weeks old significantly.Chaper IV,collect1-6weeks old AA broilers’ jejunum and caecum Get intestinal flora fingerprint and clustering analytical figure with DGGE and related software to show the influence of the GJ00007to the normal flora structure in jejunum. And the result shows that GJ00007can help to build a more complex flora structure earlier, and can establish a relatively stable one in3and5weeks old broilers GJ00007also can help to build a stable flora structure in caecum which have been stabled when the broilers are3-5years old.