Study On Soybean Meal Fermented By Multi-strains With Solid-state Bionic Way

Transform soybean meal into higher value-added soybean products with microbial fermentation technology is a hot research at home and abroad currently.The foreign industrial products have been formed. However, the domestic industry is still in the early stages in this area. In this study,soybean meal was fermented with Aspergillus niger, Bacillus subtilis, Yeast, and Lactobacillus.Use protease activity and acid soluble protein content of soybean meal as indicators,study the effect of single and mixed microbe fermentation on nutritive value of soybean meal.The best fermentation condition is found by single and mixed microbe fermentation soybean meal.On this basis, the multi-strain bionic fermented soybean meal on production performance of lactating sows.Experiment1:Growth curve of different experimental microbe and determine the inoculation time.The results showed that:Aspergillus niger,Bacillus subtilis,Yeast and Lactobacillus are the most appropriate time to seed liquid culture inoculated with24h.Experiment2:Effect of different microbe fermentation on the nutritional value of soybean meal. The results showed that different strains of the following optimal fermentation conditions, can improved nutritional value of soybean meal. Aspergillus niger: aerobic fermentation, fermentation time72h, temperature30℃, inoculum4%and water ratio1:0.9; Bacillus subtilis:aerobic fermentation, fermentation time60h, temperature30℃, inoculum3%and water ratio1:0.9; Yeast:aerobic fermentation, fermentation time48h, temperature30℃,2%inoculum and water ratio1:0.9; Lactobacillus:anaerobic digestion,fermentation time72h,temperature37℃, inoculum2%and water ratio1:1.Experiment3:Effect of multi-strain solid state bionic fermentation on the nutritional value of soybean meal. On the basis of experiment2, the two-stage fermentation pattern as follow:first aerobic fermentation,then anaerobic bionic fermentation. By optimize of multi-strain orthogonal test conditions,we found the best conditions of bionic fermentation soybean meal, they are as follows:the total fermentation time was96h. The first48hours, under the condition of30℃, use Aspergillus niger, Bacillus subtilis and Yeast aerobic fermentation.Then, the last48hours, under the condition of37℃,use Lactobacillus anaerobic bionic fermentation. Four strains the proportion of the total inoculation is10%, Aspergillus niger, Bacillus subtilis, Yeast and Lactobacillus were inoculated4%,2%,2%,2%. Four strains were inoculated before fermentation. Aerobic fermentation stage water ratio is1:0.9, anaerobic fermentation stage of biomimetic material to water ratio of1:1.2. In this condition, fermented soybean meal protease activity reached491u/g, the content of acid-soluble protein reached20.06%, the content of trypsin inhibitor decreased from12.8mg/g to0.1mg/g, the content of crude protein increased from43.75%to52.03%, the content of lactic acid reached2.13%.Experiment4:Effect of bionic fermented soybean meal on production performance of lactating sows. In conditions of optimum, bionic fermented soybean meal was produced. Lactating sows as experimental object.Control group was fed normal diet,while the other two treatments were fed experimental diets,with dried and not dried bionic fermented soybean meal substituting for20%nitrogen of soybean meal of normal diet. The results showed that:bionic fermented soybean meal could increased the number of litter size at weaning and litter weight of28-day-old(P＜0.05);shortened the interval between ablactation and oestrus(P＜0.05);significantly reduced the incidence of mastitis and constipation of lactating sows. Experimental results showed that bionic fermented soybean meal could improved performance and enhance the health status of lactating sows.