Research On The Borrelidin Of Antibacterial Activity

Soybean blight, also known as Phytophthora sojae. caused by Phytophthora root rot.,results inconsiderable loss of soybean crop yield worldwide. Every year for its generate billions of dollars ineconomic losses. From the health of soybean root extract of Phytophthora sojae resistant actinomycetes.The antibacterial activity was analyzed, A bioactivity-guided approach was then employed to isolateand determine the chemical identity of bioactive constituents with antifungal activity against P. sojae.Determine the active ingredient for borrelidin. Borrelidin of antimicrobial activity were studied in thepaper,the results are as follows:(1) Through the antibacterial activity test, determine the actinomycetes spectrum antifungalactivity of produce Borrelidin NEAU-W2. Excellent inhibition of the bacterial pathogen P. sojae、Phytophthora infestans, Alternaria solani. The inhibition of the bacterial pathogen Fusariumoxysporum， Orynespora cassiicola， Colletotrichum orbiculare， Fusarium orthoceras，Sclerotinia sclerotiorum(2) The crude extract of fermented liquid excellent inhibition of the bacterial pathogen Bacillussubtilis and Micrococcus luteus. Phytophthora sojae Kaufmann Phytophthora infestans and Alternariasolani is completely inhibited(3) Using HPLC analysis hyphae precipitation leaching content and conclusion by comparing andcrude extractings from fermented liquid its main active product for borrelidin(4) Our test used CA medium (carrot medium), the result of the half inhibitory concentration (EC50)and The best inhibitory concentration(EC95) of borrelidin for Phytophthora sojae are0.0056mg/mLand0.026mg/mL, and found it is able to completely inhibit the growth of bacterial when the concentrationof borrelidin is0.05mg/mL. It is200times higher than metalaxyl.(5) The pot experiment showed that compared the borrelidin and metalaxyl, use with less, safe,efficient, and many other advantages, indicating borrelidin fungicide potential to become a newgeneration of Phytophthora sojae.