The Promoter Of TK Gene For Equine Herpes Virus Type Ⅰ Location And Its Characteristic Analysis

Equine Herpes Virus type1(Equine Herpes Virus get1, EHV1) belong to the alpha HerpesVirus, mainly infected the horses, give priority to with respiratory symptoms and accompanied byfever, increased lymphocytes or mare third trimester miscarriage and stillbirth and other symptomsof infectious diseases. EHV1lead to the latent infections for the body, when immunity decreaseviremia and neurological symptoms. TK (Thymus Kinase) gene is a major virulence genes, EHV1missing TK genes affect herpes virus replication ability in neurons. TK gene belongs to the earlygene of the virus, its encoding product is thymidine kinase enzymes necessary to remedial ways ofsynthesis. TK disfunction of strains in the splinter cell replication capacity is very low, the nervelatent virus virulence is difficult to restore, so in TK has special significance in the study of neuralpathogenic. UL24is a early gene of EHV1, deleting UL24gene also affect the growth of virus inthe nerve cells. Existing studies have shown that herpes virus TK gene promoter is inside of UL24,but havenot make sure the TK promoter region.This experiment according to the known herpes virus TK TK gene promoter EHV1prediction-1promoter regions, and auxiliary software analysis, predict possible start regions with adjustabledevice. Based on forecast results in TK gene cloned5different lengths of upstream sequencedesign gene fragments, at the same time build free of GFP in the promoter region of the carrier,will the TK gene fragments and cloned upstream to5’UTR region, GFP, build the promoteractivity prediction system;5kinds of promoter of completed building load testing system with celltransfection, GFP expression using a fluorescence microscope and flow cytometry instrumentdetection; According to the result of predicted fragments known start-up activity, use softwareBioinformatics and Molecular Analysis Section to analysis adjust and control elements. Results: inthe above obtained results, identify three areas with promoter activity, which located in UL24gene3’end-266~+814,1,-49-100~-~1area, at the same time-266~+814area of startup activitystronger than-100-1area,-100~1area is the same as the start activity-49~1area;2has nopromoter activity area, located in UL24gene3’end of1~5’ end of the958~998and the1045area, predicted UL24gene3’end of1~998regions may have unknown transcription regulationsuppression components; EHV1of TK gene regulation the original components, TATAA andGCCCC regulatory elements as the important control component, not necessary to components,but TATAA GCCCC promoter activity area is still alone. This study for further research on TK gene transcriptional expression control and neuralpathogenesis research provides the basic data.