Protective Effects Of Kaempferol On The Acute Lung Injury Induced In Mice

Kaempferol is a flavonol present in the Zingiberaceae Kaempferia rhizome grass grass BerryBerberidaceae plant nest children seven rhizomes, Santalaceae of plants Euphorbiaceae aerial partsof the cat grass, leguminousthe licorice plant acacia trees ripe fruit. Antibacterial, cough, enzymeinhibition, mutagen activity.Mainly used for anti-cancer, inhibit fertility, anti-epileptic,anti-inflammatory, anti-oxidant, antispasmodic, anti-ulcer, choleretic, diuretic, cough. However, themechanism of kaempferol in inflammation of lung tissue damage is not yet clear. The experimentanimals with acute lung injury (ALI) model in mice induced by lipopolysaccharide, prevention andtreatment kaempferol gavage study by electron microscopy as well as timely quantitative PCR itskaempferol provide the basis for clinical applications and in-depth study on the respiratory system.A total of180mice were randomized into six groups, including model group, control group,dexamethasone (Dex) dose group and three groups of Kaempferol in high dose, medium dose, andlow dose. High dose group.Clinical symptoms, postmortem and ultrastructure were observed.Degree of lung edema, antioxidative function, inflammatory response, expression of HO-1mRNAin lung were measured to assess the effect of Kaempferol on the acute lung injury induced by LPSin mice. The following conclusions could be drawn from the experiments:1. The morphology of pathological changes were observed by transmission electron microscope,which indicated that Kaempferol possesses protective action on ALI by LPS in mice.2. Through the detection of lung wet/dry ratio, confirmed that Kaempferol could reduces the extentof lung edema induced by LPS, ameliorate the microcirculation of lung, and relieve thepathological injury of lung.3. Through the detection of SOD activity, GSH-Px activity, MDA content and DNA-proteincrosslinking rate in lung cell in lung tissue, confirmed that the Kaempferol could significantlyreduce the damage of oxidation-antioxidant balance induced by LPS, make the lung cellDNA-protein crosslinking coefficient close to normal. It confirmed that the antioxidation ofKaempferol plays an important role in reducing the acute lung injury induced by LPS in mice.4. Through the detection of MPO activity and the expression of NF-κB, IL-1β, PTGEs, HO-1,iNOS, COX-2, and TNF-α mRNA in lung, confirmed that Kaempferol could suppress MPO activity and reduce the expression of inflammation-stimulating factors, possesses protective action on theALI induced by LPS in mice.5. Through the detection of HO-1mRNA expression which detected by RT-PCR, confirmed thatKaempferol could advance HO-1mRNA expression. It demonstrated that Kaempferol couldenhance endogenous protection of body, thus possesses protective action on the ALI induced byLPS in mice.This research shows that Kaempferol could decrease the ALI induced by LPS through thedetection on the inflammatory response, antioxidative function and expression of HO-1mRNA inlung. It provides the experimental basis for clinical applying and the scientific theoreticalfoundation of the Kaempferol.