Characteristics Analysis Of A Lipid-transfer Protein Gene GmLTP3in Glycine Max

Lipid transfer proteins (Lt-ps) play important roles in lipid transportation, plant reproduction and development and adaptation to adverse environmental stresses. The activity of fatty acid biosynthesis enzyme and oil content were controlled by the spatial and temporal expression level of LTP gene.In this study, a gene which have94%identity with the SiLTP3was cloned and named as GmLTP3. GmLTP3encodes a LTP that have99%identity with the known Glycine max lipid transfer protein. The transcriptional level of GmLTP3was quantitatively determined using realtime-PCR, it was expressed in almost all the tissues, such as root, stem, leave, flower and seed. It was highly expressed in alabastrum,7-days old stem and3-days germinating seed, while it was lowly expressed in root and leave. When treated by abiotic stress, such as IAA, ABA, PEG and NaCl, the expression level was down-regulated after treated2hours later, but was up-regulated after treated12hours later, while it is down-regulated consistently after treated by cold stress.