Classification Of Musa Using Molecular Identification

China is one of the main producer of bananas. However, the reasearch of banana breeding is slow, and the main banana production is based on the triploid cultivars introduced from others countries. Geographic origins and evolvements of cultivated bananas is subject of debates.Based on these information, the study was aimed to sequence 2 non-coding chloroplast DNA and 1 nuclear internal transcribed spacer (ITS) sequences from multiple wild and cultivated banana accessions and assess molecular variability and potential phylogenetic relationship in Musa.The main results and conclusions were as follows:1. Using non-coding chloroplast DNA sequences, wild accessions from Sect. Australimusa were clearly distinguished from other three sections. Sect. Australimusa origined from Papua New Guinea, which has a relatively unattached geographic encironment. It is reasable that the genome designation was named as "T" to distinguish from "A" and "B" genome by some reasearchers. The results in this study are similar to the former.2. M. marges elargies (Sect. Eumusa) and M. beccarii (Sect. Callimusa) seemed to have a close relationship with Sect. Rhodochlamys based on the high homology in the sequences. But it is not conforming to the results of Gawel. Gawel et al (1992) reported that M. beccarii and M.acuminata had a highly close relationship using RFLP.3. The sequence of M. yunnanensis was more complicated. It had higher homology in the sequence with Sect. Rhodochlamys. But the NJ tree showed that M. yunnanensis was not grouped with the accessions of Sect. Rhodochlamys.4. There were obviously different sequence in M.schizocarpa compared with the sequences of the accesstions with "A" and "B" genome. This was consisted with Ude et al.(2002).5. At intraspecific level, two sequences from cpDNA can separate the accessions of M. balbisiana into two groups, and the classification of each sequence for the accessions is consisted with each other.6. The cultivated accessions:M.niyarma yik, M.pisang sapon, M.kungor, M.odwa, M.prata, M. safet velchi, and M.djum tau were grouped with M. a. zebrina based on the analysis of two sequences from cpDNA. We may conclude that these accessions have a maternal M. acuminata subsp.zebrina origin. 7. From our results, non-coding cpDNA sequences are useful to lineage assessment and origins of Musa.