Simiaojunyi Ointment Preparation And Comparison Research Of Give This Medicine Between Through The Skin And Through The Mucosal

Objective:In order to maximize the treatment of postoperative pain and promote healing of hemorrhoids effect, the project will be made into ointments, and compared with the powder, from the preparation process, quality control, and other aspects of the transdermal permeability of a comparative study for the development of hemorrhoids postoperative pain and promote wound healing ointment for providing a scientific basis for traditional Chinese medicine.Methods:(1) Use the Supercritical CO2 method extracted of Volatile Oil from the pepper, Comparison of steam distillation and supercritical CO2 method which can extracted more volatile oil from the pepper. (2) Use the Supercritical CO2 method extracted Tetrahydropalmatine or extracted Tetrahydropalmatine by ethanol and Chloroform from the Corydalis, Comparison the two method who can extracted more Tetrahydropalmatine from the Corydalis. (3) Used for the phenomenex C18-ODS column column, the mobile phase was methanol-0.1% phosphoric acid solution (triethylamine adjusted pH=6.0), linear gradient elution:0 min～20 min,60% methanol; 20 min～30 min,65% methanol; a flow rate of 1.0 mL/min, detection wavelength was 280 nm, and the establishment of Corydalis extract ointment in the Determination of tetrahydropalmatine. (4) Consistency with levels of matrix effects of different or the same matrix effect of different proportions of shape, selected the best cream formula and process parameters of matrix. (5) Verifying the percutaneous absorption speed of ointment is more than Powder by excised skin method.Results:(1) References in the last decade pepper herbs selected supercritical CO2 extraction of the optimum conditions:Size:Medium (24 mesh); cosolvent ethanol concentration:75%(5% addition level quality of raw material); extraction pressure:30 MPa; extraction temperature:40℃; extraction time:2 h. In supercritical CO2 extraction of volatile oil by adding 6 times the amount of water, will be the place essential oil extractor with refluxing 8 h, parallel to the 3 copies of the results of volatile oil extraction rate of 0.22%; weigh a certain amount of pepper meal ingredients, plus 6 times the amount of water, reflux extraction 8 h, parallel to the 3 copies of the results of volatile oil extraction rate was 1.25%; The experimental results show that the steam distillation of the volatile oil extraction rate of supercritical CO2 extraction method is Extraction of volatile oil extraction rate of 5.68 times. Therefore, extracted by steam distillation is better than volatile oil extraction with supercritical CO2 extraction of volatile oil. (2) References in the last ten years selected herbs Corydalis supercritical CO2 extraction of the optimum conditions:Size:Medium (24 mesh); cosolvent ethanol concentration:70%; extraction pressure:20 MPa; extraction temperature: 55～50～45℃; CO2 flow rate:30 Kg/h; fumarate herbs soaked with ethanol 24 h; extraction time:1 h. References in the last decade with selected herbs Corydalis ethanol, chloroform extraction to extract the optimal conditions Tetrahydropalmatine:75% ethanol extract 2:Part 1 plus 6 times the amount of alcohol, soaked in 0.5 h, reflux extracted 1.5 h, 4 times 2nd plus the amount of alcohol, refluxed 1.5 h. Filtration, recycling ethanol concentration may fumarate dry extract. To 100 mL 1% hydrochloric acid solution by adding ethanol extract of Corydalis, fully ground after filtration, precipitation continued to increase from 1% dilute hydrochloric acid solution filtered. Merge two filtrate, ammonia filtrate adjusted around pH=9, an equal volume of chloroform extract 3 times, was chloroform solution, vacuum recovery of chloroform, that was rough Corydalis alkaloids. Two ways to extract extracted by high performance liquid chromatography tetrahydropalmatine content, the results show that the supercritical CO2 extraction method is superior to ethanol, chloroform extraction method. (3) Using HPLC technique, we detected the samples by the C18-ODS column of Phenomenex company under the wavelength at 280nm, the chromatographic conditions were phenomenex C18-ODS column, mobile phase of methanol-water (0 min～10 min 60% A; 20 min～30 min,65% A), flow rate of 1.0mL/min and detection wavelength at 280nm. (4) Ointment of the matrix by the PEG 4000 40%:60% composed of polyethylene glycol 400, The two kinds of polyethylene glycol, In a water bath heated to 65℃, Remove the water heating, Join research into fine powder (over 120 mesh sieve) and Panax notoginseng extracts of Corydalis, When the temperature dropped to room temperature, Added and the volatile oil of myrrh, Stir until condensation. (5) Transdermal ointment, through the mucous membrane faster than spraying (P<0.05), Faster than the penetration through the mucosa (P <0.05), Penetration, penetration through the mucosa is much higher than the cumulative four wonderful Jolly spraying(P<0.05), The cumulative penetration through the mucosa is much higher than the penetration(P< 0.05),The Skin reserve proportion rate of SIMIAOJUNYI ointment is 0.4787%, The Skin reserve proportion rate of SIMIAOJUNYI Powder is 0.1783%, The Mucosal reserve proportion rate of SIMIAOJUNYI ointment is 0.5386%. The ointments storage capacity of the skin is higher than the powder. Through the mucosa is higher than transdermal. Conclusions:The results prepared in accordance with four wonderful Caramel ointment process is feasible, quality control, product stability. And that the four wonderful Caramel cream used for clinical effect is significant, doctors and patients to give a higher evaluation of the preparations.