Effect Of H₂S On Intracellular Ca²⁺ Concentration In HSCs Under Oxidative Stress Induced By H₂O₂

Objective The purpose of this paper was to discuss the effects of H₂S on ratsprimary hepatic stellate cells （HSCs） proliferation and Ca²⁺ concentration under theoxidative stress induced by H₂O₂and to explore the possible mechanism.Methods Models of oxidative stress were induced by exposing rats primary HSCsto H₂O₂. HSCs were loaded by Flu-3/AM. Then the cells were treated with Glyburide（a kind of KATPchannel inhibitor） and different concentrations of NaSH （a kind ofH₂S donor）. The Ca²⁺ concentration were examined using laser scanning confocalmicroscope （LSCM）. The HSCs proliferation were measured using CCK-8assay.Results Low concentration of H₂S （100μmo/L NaSH） significantly decreased theconcentration of Ca²⁺ and inhibited the HSCs proliferation （p<0.05）. KATPchannelinhibitor （Glyburide） could block the effect of H₂S. High concentration of H₂S（1mmo/L NaSH） significantly increased the concentration of Ca²⁺ and promoted theHSCs proliferation （p<0.05）.Conclusion Low concentration of H₂S decreases the concentration of Ca²⁺ andinhibits the HSCs proliferation by stimulating the KATPchannels in HSCs. However,high concentration of H₂S increases the concentration of Ca²⁺ and promotes the HSCsproliferation.