Production And Characterization Of Virus-like Particles Of Two Human Enteroviruses

Enterovirus belongs to the Picornaviridae (small RNA) virus family, includingcoxsackie virus (Cox), new enterovirus (EV68-102), poliovirus (PV) and echovirus.At present, EV72was reclassified and renamed as hepatitis virus A (HAV). HAV isa gastro-intestinal infectious diseases leading to liver damage or failure. Hepatitis Ashows a high incidence in China, which attracts a great attention from Chinesegovernment. Live attenuated and inactivated hepatitis A vaccine are widely used inChina, which play an important role in HAV prevention. Coxsackie virus A16(CoxA16) is one of the major etiological agents of hand, foot and mouth disease (HFMD),which can lead to herpes pharyngeal angina, meningitis and other serious diseasesthat threate the health of children. During the first quarter of2012, Cox A16infection-related HFMD was reported to increase by216.67percent higher than thesame period of last year. There is still no prophylactic vaccine available on market toprevent Cox A16infection. Therefore, we try to produce the virus-like particle (VLP)of HAV and Cox A16to made genetically recombinant vaccines based on the VLPof HAV and Cox A16.In this study, two different methods were used to produce the VLP of HAV andCox A16virus. Baculovirus expression system was adopted to produce HAV VLP.Electron microscope analysis showed that co-expression of P1and3ABC proteins ininsect cells resulted in the generation of HAV VLP. Saccharomyces cerevisiae wasadopted as expression system to produce Cox A16VLP. Electron microscopeanalysis showed that co-expression of P1and3CD protein of Cox A16led to theproduction of Cox A16VLP in yeast.The VLPs of HAV and Cox A16were produced in this study, which maypotentially encourage the development of genetically recombinant vaccine againstthe infection of HAV and Cox A16.