| Objective To discuss the survivorship of rat mesenchymal stemcells(rMSCs) and the expression of human brain-derivedneurotrophic factor (hBDNF) protein after transplantation ofthe Hbdnf-modified rMSCs (hBDNF-rMSCs) to the adult rats withspinal cord injury (SCI) and discuss the effect of hBDNF-rMSCson the apoptosis of rat neural cells.Methods A total of240adult Sprague-Dawley（SD） rats wererandomly divided into four groups including sham operationgroup,SCI group,hBDNF-rMSCs transplantation group andAD-EGFP-rMSCs transplantation group.The SCI model was made byusing the modified Allen’s technique. At day7after thoracicSCI, in hBDNF-rMSCs group, AD-EGFP-rMSCs group and SCI group,the same amount of hBDNF genetically modified rMSCs suspension,Ad-EGFP genetically modified rMSCs suspension and phosphatebuffered saline(PBS) were injected into the subarachnoid spacefrom L4-5intervertebral space respectively. Then, the rats were sacrificed and the spinal cord was removed at different timepoints(at day1,2,3,7and14). The viability and distributingof rMSCs with enhanced green fluorescent protein gene in theinjured spinal cord were observed by fluorescentmicroscope,the level of hBDNF expression in the injured spinalcord were analyzed with Western blotting. The change ofapotosis was observed with TUNEL method and eosin(HE) staining.Results (1) The fluorescent microscope showed: In hBDNF-rMSCsand Ad-EGFP-rMSCs groups,the green fluorescence expression inrMSCs were observed．(2)Western blotting showed:The hBDNFexpression was examined in hBDNF-rMSCs group by Westernblotting. The expression of hBDNF could be detected at day2after transplantation,reached the highest at day7and thendecreased gradually.(3)HE stain displayed:The pathologicalchange of the spinal cord tissue in sham operation group wassimilar to the normal,while that in hBDNF-rMSCs group visiblyameliorated better than in injury group.(4)TUNEL test showed:At2,3,7,14day after transplantation,the number ofTUNEL-positive cells of hBDNF-rMSCs group was thesmallest,while the number of TUNEL-positive cells ofAD-EGFP-rMSCs group was smaller than that of SCI group.Conclusion Transplanted hBDNF genetically modified rMSCs are able to survive and assemble at the injured area of spinal cord;Transplanted hBDNF genetically modified rMSCs are able toexpress hBDNF there； hBDNF genetically modified rMSCstransplantation may inhibit apoptosis of neural cells in spinalcord tissue after SCI. |
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