The Epidemiological Study On The Association Of X-ray Repair Cross Complementing Gene Polymorphisms And Environmental Factors With The Risk Of Non-small-cell Lung Cancer

[Objective]To investigate the environmental factors of non-small-cell lung cancer (includethree histological types) and the relationship between X-ray repair crosscomplementing gene polymorphisms and the susceptibility of non-small-cell lungcancer, and the combined effects in the development of carcinoma.[Methods]A case-control study was conducted with890patients and1055control subjects.The patients who were confirmed by bronchoscopy and pathologic diagnosis wererecruited from the First Clinical Medical College, the Affiliated Union Hospital ofFujian Medical University and Fuzhou General Hospital. The healthy control subjectswho were frequency-matched to the cases on age(±1year) and sex were enrolled fromhospital visitors and community population. Face-to-face interview was performed toevery subject.5ml blood sample from each patient were collected. The collection ofsamples among control subjects was accomplished by using OrageneTMDNAself-collection kit. The genomic DNA was extracted from all blood and saliva samples.The genotypes of XRCC2、XRCC3and XRCC4polymorphisms were determined bySequenom platform and PCR-RFLP. EpiData3.0was used to collect data from fieldinvestigation and SPSS19.0to analyse the data, except GMDRv0.9software toestimate the interaction effects.[Results]1. The major risk factors of non-small-cell lung cancer (include three histologicaltypes) include: air pollution near resident, insufficient ventilation in resident, cooking oil fume,smoking, ETS at home/work, history of lung disease and introverted character. The protective factors included: control cooking temperature, take fresh fruit and drinking tea.2. No association between the SNP rs6464268in XRCC2and the susceptibility ofnon-small-cell lung cancer (include three histological types) was observed.3. The SNP rs1799796in XRCC3connect with the risk of squamous carcinoma oflung.4. The SNP rs1805377and rs9293337in XRCC4connect with the risk ofnon-small-cell lung cancer and lung adenocarcinoma.5. The major risk factors of lung adenocarcinoma among nonsmokers include cookingoil fume, consumption of fruit (<3per week), rs1799796(A/A) and rs1805377(A/A).[Conclusion]There are different environmental factors or genetic polymorphisms amongnon-small-cell lung cancer and three histological types of lung cancer, control andprevention for lung cancer should be based on the characteristics of differenthistological types.