| The genesis of gastric cancer is a multi-factor, multi-gene, and multi-step processinvolving many related genes and proteins. The plasma membrane proteins play animportant role in the process of life activities and in the field of treatment. Therefore,it’s important to study the plasma membrane proteomics about gastric cancer thatwould help us to understand the metastasis mechanism more and open up new idea ofdiscovery pharmaceutical research. In our research, fluorescein/biotin double-labeling—avidin enrichment technology is adopted to purify the plasma membrane proteins ofgastric cancer. And then, the shot-gun-MS was applied to get a visualized, abundantand intact plasma membrane protein spectrum which has a close relation to infiltrationand metastasis of gastric cancer. After analysing the biological function of thoseprotein,we find that there are four proteins have been proved to involve in the processof metastasis of gastric cancer, including HSP90, R-cadherin, AIFM1and S100A9.R-cadherin is a number of the cadherin family encoded by CDH4. We verify thatR-cadherin is expressed in a cellular level and tissue levels, and there is much lowerexpression in gastric cancer. In order to confirm the function of R-Cadherin in gastriccancer, CDH4was cloned from the normal gastric epithelial tissue and constructed therecombinant over-expression plasmid which was transfected into gastric cancer celllines BGC-823, following picked out the stable transfected cell line further. Throughthe grow curve, wound healing assay and transwell cells experiment, we demonstratethat CDH4could inhibit the proliferation capacity and decrease invasiveness andmigration capability in gastric cancer cells.Above all, the expression differences of CDH4/R-cadherin is the molecular basisof biological behaviors of malignant gastric cancer cells; besides, CDH4is a potentialtumor inhibitory factor of gastric cancer, and R-cadherin would be a target ofanti-tumor drug. |
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