Study On The Relationship Between Intrahepatic Quasispecies Distribution Of Hepatitis B Virus After Nucleoside Analogue Withdraw:a Prospective Study

Objective: To investigate the intrahepatic quasispecies distribution ofhepatitis B virus of hepatitis B patients who received nucleoside analoguetreatment and reach the the standard of drug withdraw, to investigate themolecular virology mechanism of end-of-dose failure.Methods:A total of10chronic hepatitis B patients who received nucleosideanalogue treatment and reach the the standard of drug withdraw wereenrollede.The HBV DNA was extracted from the liver when drugwithdrawl,thereverse transcriptase gene was amplified by PCR assay,then cloned andsequenced. All patients were fllowed up for24weeks by per week, thendivieded into last response group and replase group according to thedifferent responses. The quasispecies complexity and diversity of HBV werecompared between the two proups by bioinformatics methods. Thephylogenetic trees were contructed by neighbor-joining method.Result:The serum HBsAg of relapse group was significantly higher than thelast response group (P=0.028).The inflammation and fibrosis of liver andthe HBsAg of immunonistochemisty were not significantly different betweenthe two groups(P>0.05). The quasispecies complexity and diversity were notsignificantly different between the two groups(P>0.05).The branch lengthof sequences from the two groups is similar.Conclusion: The serum HBsAg on the time of drug withdrawl is more lower,The probability of recurrence is more smaller. Histopathology of liveris not a good predictal index for relapse after nucleoside analogue withdraw. The quasispecies complexity and diversity actions as a predictal index forreplase after nucleoside analogue withdraw is not sure, depended on thefurther research.