| Objective:To detect the expression of TCTP in βTC3cells treated withdifferent concentrations of FFA and in the pancreas of male SD rats treated withhigh fat diet；Then to investigate the intervention effct of Lira on the change ofTCTP induced by FFA at cell level.Methods：1． βTC3cells were exposed to different concentrations of FFA (0,0.125,0.25,0.5and1.0mmol/L). Western Blot analysis was used to determine theexpression of TCTP in βTC3cells after24hours. Afterwards, βTC3cells werepreincubated with different concentrations of Lira（0,0,0.5,1mg/L）for6hours,and different concentrations of FFA（0,1,1,1mmol/L）were then added and thecells were incubated for another24hours. The expression of TCTP and active caspase-3were detected.2．12male SD rats were divided randomly into the normal diet (ND) groupand high fat diet (HFD) group, which were feeded with normal diet and high fatdiet respectively. After20weeks, the level of blood lipid was detected toascertain the successful establishment of the rat model of high fat diet. After therats were put to death, real time PCR and Western Blot were employed todetermine the expression of TCTP in the pancreas at the level of mRNA andprotein.Results:1． After the cells were incubated with FFA of different concentrations for24hours, compared with the control group, the expression of TCTP in βTC3cells in both groups treated with0.5mmol/L FFA and1mmol/L FFA decreased(P<0.05); Compared with the group with1mmol/L FFA, the expression ofTCTP increased (P<0.05) and expression of active caspase-3decreased (P<0.05)in the group of0.5mg/L Lira+1mmol/L FFA and the group of1mg/L Lira+1mmol/L FFA, and these two groups were statistically different (P<0.05).2． Compared with the ND group, the rats in the HFD group obtainedhigher levels of triglyceride （HFD1.18±0.06vs ND0.89±0.03, mmol/L, P＜0.01) and total cholesterol （HFD2.37±0.13vs ND1.77±0.12, mmol/L, P＜0.01) in serum. No significant differences of expreesion were seen in pancreaticTCTP mRNA and protein between these two groups.Conclusions:1． The expression of TCTP in βTC3cells is down-regulated byadministration of FFA of certain concentration, while Lira helps to relieve this down-regulation and TCTP may be involved in the anti-apoptosis process ofLira.2． The rat model of high fat diet was successfully established. And therewas no statiscal difference in the pancreatic TCTP mRNA and protein betweenthe two groups. Objective:To detect the expression of PERK in βTC3cells exposed to differentconcentrations of FFA and the intervention effect of Lira on it.Methods:βTC3cells studied in the experiment were treated with differentconcentrations of FFA (0,0.125,0.25,0.5and1.0mmol/L). Western Blotanalysis was used to determine the expression of PERK in βTC3cells after24hours. Afterwards, βTC3cells were preincubated with different concentrationsof Lira (0,0,0.5,1mg/L) for6hours, and different concentrations of FFA (0,1,1,1mmol/L) were then added and the cells were incubated for another24hours.The expression of PERK was detected.Results:1． After the cells were incubated with FFA of different concentrations for24hours, compared with the control group, the expression of PERK in βTC3cells treated with1mmol/L FFA increased (P<0.05). 2． Compared with the group with1mmol/L FFA, the expression of PERKdecreased (P<0.05) in the group with0.5mg/L Lira+1mmol/L FFA and thegroup with1mg/L Lira+1mmol/L FFA, and these two groups were statisticallydifferent (P<0.05).Conclusion:The expression of PERK in βTC3cells is up-regulated by administration ofFFA of certain concentration in vitro, while Lira can reverse this response tosome extent, by which we guess partly inhibits endoplasmic reticulum stress. |
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