The Expression And Significance Of Snail And E-cadherin In Clear Cell Renal Cell Carcinoma

Objective To study the expression of transcriptional factor Snail and adhesionfactor E-cadherin in clear cell renal cell carcinoma (CCRCC) and evaluate theircorrelation with their pathological grading, clinical stage, invasion and metastases oftumor.Methods Sixty-nine specimens of renal cell carcinoma tissues were collectedfrom the First Affiliated Hospital of Henan scientific and technical university fromOctober,2010to June,2011. All specimens were identified to be clear cell renal cellcarcinoma by histopathologic examination after surgery. According to the TNMstaging system in American Joint Committee on Cancer in2002,36cases were in T1,20cases were in T2,10cases were in T3and3cases were in T4. According to theFuhrman grading system,11cases were in G1,30cases were in G2,20cases were inG3,5cases were in G4. In the meanwhile,58cases of the para-cancerous mucosa and10cases of normal renal tissues were acquired as a comparison. The expression ofSnail and E-cadherin protein was detected by using immunohistochemicalstreptavidin-peroxidase method in CCRCC tissue, para-cancerous mucosa and normalrenal tissue. And we will analyze relationship between the expression of Snail and E-cadherin and clinical pathology factors, as well as the correlation between Snail and E-cadherin expression.Results The Snail stain located on nucleus, but having no expression oncytoplasm and membrane. The positive rate of Snail in CCRCC was82.7%, which washigher than para-cancerous mucosa(43.1%)and normal renal tissue(10.0％), and thedifference was remarkable and significant(P<0.001). The expression of Snail wassignificantly related to the differential degree, clinical stage, depth of tumor invasionand distant metastasis(P<0.05). The E-cadherin stain located on cytoplasm and (or)membrane, but having no expression on nucleus. The positive rate of E-cadherin inCCRCC was31.9%, which was lower than para-cancerous mucosa(91.5%) and normal renal tissue(100％), and the difference was remarkable and significant(P<0.001). Theexpression of E-cadherin was significantly related with the differential degree, clinicalstage, depth of tumor invasion and distant metastasis (P<0.05). But the expression ofE-cadherin and Snail were not related with the age and sexual of patient. There wasnegative correlation between expression of E-cadherin and Snail in CCRCC.Conclusion1、The positive expression rate of Snail gradually increased innormal renal tissue, para-cancerous mucosa and CCRCC tissue, and that wassignificantly related with the differential degree, clinical stage, depth of tumorinvasion and distant metastasis. The result show that the increased positive expressionrate of Snail was closely related to progression of CCRCC and could promotedinvasion and metastasis.2、The positive expression rate of E-cadherin gradually decline in normal renaltissue, para-cancerous mucosa and CCRCC tissue, and that was significantly relatedwith the differential degree, clinical stage, depth of tumor invasion and distantmetastasis. The result shows that the degressive positive expression rate of Snail wasclosely related to progression of CCRCC and could promoted invasion and metastasis.3、The overexpression of Snail and decreased expression of E-cadherin might beimportant biological markers for invasion and metastasis of CCRCC. There wasnegative correlation between expression of E-cadherin and Snail in CCRCC. Thecombined detection of E-cadherin and Snail has far-reaching significance onpredicting the invasion and metastasis in CCRCC.