The Effect Of Hyaluronan On The Motility Of Skin Dermal Fibroblasts In Nanofibrous Scaffolds

The applications of nanofibrous scaffolds and native extracellular matrix arepromising approaches for tissue regeneration. However, one limitation of electrospunnanofibrous scaffolds is the relatively poor cell infiltration into3D structure, whichretards matrix remolding and tissue regeneration. In this study, we focus on hyaluronanas a native ECM regulates cellular motility in nanofibrous scaffolds. This articlecontains detailed contents as follows:①PCL、PCL/HA5:1and5:2nanofibrous scaffolds were generated byelectrospinning with HFIP and FA as the solvents. The nanofibers were characterizedfor various physicochemical properties，such as SEM、TEM、FTIR、Contact Angle andMechanical testing. PCL/HA blended nanofibers could provide mechanically stablestructure for tissue engineering application.②The effect of HA addition to PCL on cell proliferation and collagen I, IIIexpressions were investigated. Cells in the PCL/HA fiber membrane showed goodproliferation, suggesting that the materials had no toxic to cells and suitable for cellgrowth.③To determine the contribution of HA component response to cell infiltrationand migration into the nanofibrous, this artical used two experimental models-Transwell system and Time-lapse microscope. The observation of living cellsmovement revealed that2D HA coating and3D HA-based scaffolds had differenteffects on cell migration speed and direction. Cells on1%HA coating moved in moredeliberate and precise orientation. These cells had large increase of filopodia-likeprocesses and higher CD44expressions. HA-based scaffolds significantly enhanced cellinfiltration and migration in vitro. This phenomenon may influences by the variation incell adhesion receptors formation and distribution. Furthermore, we confirmed thatHA/CD44interactions can activate TGF-β/MMP-2signaling pathway that promote cellmotility. These findings suggest the functions of HA on cell motility in nanofibrousscaffolds and provide insightful understanding of the possible application of HA-basedscaffolds for skin tissue regeneration.④From the hematoxylin and eosin (HE) staining, we found HA-based scaffoldssignificantly enhanced cell infiltration after implanted the subcutaneous of SD rats for4weeks. Our experimental demonstrated that: electrospinning PCL/HA nanofiber scaffoldwhich is similar to the extracellular matrix structure has a good surface topology, idealphysical properties and good biocompatibility, providing a good external environmentfor cell infiltration and migration. These results provide a theoretical and experimentalbasis for the materials using for tissue engineering, tissue repair, wound dressings.