The Research Of Protection Mechanism Of HGF In Intracerebral Hemorrhage Of Rats

Object:To investigate the expression of HGF surrounding hematoma in intracerebralhemorrhage, and the effect of HGF in microvascular regeneration,neurons apoptosisand learning and memory function recovery.Methods:1.30Healthy male SD rats were divided into six groups randomly, intracerebralhemorrhage groups:6h,24h,3d,7d, and14d after intracerebral hemorrhage;Sham-operated group for the control group. The neural function defect scale changeof rat were obversed, and rats were killed at each time point. Pathological morphologychange detect by HE Staining. Immunohistochemistry method were used to detect theexpression of HGF.2. The rats were divided into model group, HGF intervention group and NSintervention group after ICH model were established. The three groups were groupedby different phases divided into six sub-group. that was6h,24h,3d,7d,14d,28d. InHGF intervention group HGF (15μl) was injected into the lateral ventricle, NSintervention group NS(15μl) was injected into the lateral ventricle.The sub-group of28d for the experiment of morris water maze, the other rats were killed at differentphases. The tissue of rat brain should be reservesd. The expression of Factor Ⅷwhich marked microvessels were detected by immunohistochemical staining and theexpression of apoptotic cells detected by TUNEL method. Results:1.HGF expression were appeared when ICH model was established6hours,significant increase at three days, the expression were intensive at7days, andthe expression were down slowly at2weeks. Each point in time of HGF expressionmainly in the hematoma peripheral zone.2.Factor Ⅷ immunohistochemical results:.(1).The expression of Factor Ⅷ mark microvessels positive cells in6h wereincreasing, and the expression were rised gradually, the peak of expression were in7d. The expression were decreased in14d. Compared ICH after24h,3d,7d,14dgroups with Sham-operated group, the distinction bears significance in statistics(P<0.01).(2).Compared the number of factor Ⅷ positive cell in HGF group, NSintervention group and model group, the distinction bears significance in statistics(P<0.05), and the distinction between NS intervention group with model groupbears no significance in statistics (P>0.05).3.TUNEL staining results:The numbers of apoptotic cells of HGF intervention group was significantlyreduced than NS intervention group and model group at each point in time, thedistinction bears significance in statistics (P<0.05).4.Water maze: experiment results:In the sixth day experiments, the escape incubation period of28d-HGFintervention groupdistribution (26.52+/-2.932s, n=4), and there are significantdifferences in statistics with28d-NS intervention group and28d-ICH model group(P<0.05).Conclusion:1. The expression of HGF was raised in the brain tissue after intracerebral hemorrhage;2. HGF can promote microvascular regeneration;3. HGF can inhibit the apoptosis of neurons after intracerebral hemorrhage;4. The damaged learning and memory function can be improved in intracerebralhemorrhage after HGF intervention.